biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
2D9, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FADS1(3992)
General description
Anti-FADS1, mouse monoclonal, clone 2D9, recognizes the ~50-55 kDa FADS1 n HeLa cells and human skeletal muscle tissue. It is validated for use in ELISA, WB, and IHC (paraffin sections).
Purified mouse monoclonal antibody. Recognizes the ~50-55 kDa FADS1 protein.
Recognizes the ~50-55 kDa FADS1 protein in HeLa cells and human skeletal muscle tissue.
Immunogen
A recombinant polypeptide corresponding to amino acids 1-100 of human FADS1, expressed as a GST fusion protein
Application


ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, Human skeletal muscle tissue
HeLa cells, Human skeletal muscle tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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