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ST1622

Anti-LEPRE1 Mouse mAb (3C7)

Name: Anti-LEPRE1 Mouse mAb (3C7)
Brand: MM

liquid, clone 3C7, Calbiochem^®

Synonym(s):

Anti-Leucine Proline-Enriched Proteoglycan 1, Anti-Leprecan 1

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

3C7, monoclonal

Species reactivity:

human

Application:

—

Citations:

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Properties

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

3C7, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (6 µg/mL))

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~90 kDa LEPRE1 protein.

Recognizes the ~90 kDa LEPRE1 protein in HeLa cells and human esophagus tissue.

This Anti-LEPRE1 Mouse mAb (3C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of LEPRE1.

Immunogen

A recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (6 g/ml)

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HeLa cells, Human esophagus tissue

Other Notes

Choi, JW, et al. 2009. PLoS Genet.5, e1000750.
Cabral, W,A., et al. 2007. Nat Genet.39, 359.

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c