UFC205024
Amicon® Ultra Centrifugal Filter, 50 kDa MWCO
sample volume 2 mL, regenerated cellulose membrane, MWCO 50 kDa
Synonym(s):
Centrifugal concentrator, 50 kDa MWCO, 2 mL sample volume
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About This Item
material
polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
2 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
119.7 mm
diam.
15.9 mm
filtration area
1 cm2
working volume
≤2 mL
2 mL
pore size
50 kDa MWCO
greener alternative category
shipped in
ambient
General description
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 2 mL filters deliver the highest recovery with shortest spin times.
Application
- Ultrafiltration with 50,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates.
- Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 50 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 2 mL down to 15-70 μL
- 98% sample recovery
- Fast processing time: 10 to 30-minute spin times
- Engineered dead stop
- Reverse spin capability provides consistent recoveries
Physical form
Other Notes
Legal Information
Certificates of Analysis (COA)
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Articles
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
本页内容讨论了使用超滤膜进行蛋白样品制备的原理和优势。应用包括溶剂置换、蛋白样品浓缩或纯化。
Related Content
浓缩你的蛋白质，别失去它。使用Amicon® Ultra过滤器进行浓缩，能可靠回收蛋白质。
"The Amicon® Ultra 2 mL filter provides a fast and easy way to concentrate, desalt and exchange thebuffer for both proteins and nucleic acids. Now you can concentrate 2 mL down to 15 – 70 μL in anAmicon® Ultra centrifugal device."
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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