As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

Nicotinamide adenine dinucleotide (NADH) has been used in:

• nicotinamide adenine dinucleotide (NAD⁺) /NADH cycling assay.
• adenosine triphosphate (ATP)ase assay.
• pyruvate kinase enzyme assay.

NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD⁺/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

Packaged by solid weight.

Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C.

Contaminants: Relative reaction with LDH: 1.00 ± 0.05 (referred to a standard)
Organic solvents: <4% (GC)

Absorbance coefficients: 14.3 x 10³ l x mol^-1 x cm^-1 at 260 nm, 6.18 x 10³ l x mol^-1 x cm^-1 at Hg 334 nm, 6.3 x 10³ l x mol^-1 x cm^-1 at 340 nm, 3.4 x 10³ l x mol^-1 x cm^-1 at Hg 365 nm

For life science research only. Not for use in diagnostic procedures.