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Merck
CN

10107735001

Roche

NADH

100% (dry weight), amorphous powder, pkg of 500 mg

Synonym(s):

β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate, β-DPNH, β-NADH, DPNH, Diphosphopyridine nucleotide, reduced form, NADH

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About This Item

Empirical Formula (Hill Notation):
C21H27N7Na2O14P2 · xH2O
CAS Number:
606-68-8
Molecular Weight:
709.40 (anhydrous basis)
UNSPSC Code:
12352204
PubChem Substance ID:
329748895
MDL number:
MFCD03791273

Key Documents

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InChI

1S/C21H29N7O14P2.2Na.H2O/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33;;;/h1,3-4,7-8,10-11,13-16,20-21,29-32H,2,5-6H2,(H2,23,33)(H,34,35)(H,36,37)(H2,22,24,25);;;1H2/q;2*+1;/p-2/t10-,11-,13-,14-,15-,16-,20-,21-;;;/m1.../s1

SMILES string

O.[Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@@H]2O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O

InChI key

FWBNCIFELNNJCX-UHOVGGJYSA-L

description

Grade I, disodium salt

assay

100% (dry weight)

form

amorphous powder

mol wt

Mr 665.4 (NADH), Mr 709.4 (NADH-Na2)

packaging

pkg of 500 mg

manufacturer/tradename

Roche

pH

7.5 (100 mg/mL in water, ±0.5), 7.5 ± 0.5 (100 mg/mL in water)

λmax

260 and 340 nm

shipped in

wet ice

storage temp.

2-8°C

Application

As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
Nicotinamide adenine dinucleotide (NADH) has been used in:
  • nicotinamide adenine dinucleotide (NAD+) /NADH cycling assay.
  • adenosine triphosphate (ATP)ase assay.
  • pyruvate kinase enzyme assay.

Biochem/physiol Actions

NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

Packaging

Packaged by solid weight.

Preparation Note

Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C.

Analysis Note

Contaminants: Relative reaction with LDH: 1.00 ± 0.05 (referred to a standard)
Organic solvents: <4% (GC)
Absorbance coefficients: 14.3 x 103 l x mol-1 x cm-1 at 260 nm, 6.18 x 103 l x mol-1 x cm-1 at Hg 334 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm, 3.4 x 103 l x mol-1 x cm-1 at Hg 365 nm

Other Notes

Shipping conditions may vary
For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
50640921
50640920
46675921
46589027
45507629

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Isotopically nonstationary 13C flux analysis of cyanobacterial isobutyraldehyde production.
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Control of Mitochondrial pH by Uncoupling Protein 4 in Astrocytes Promotes Neuronal Survival.
Lambert HP, et al.
The Journal of Biological Chemistry, 289(45), 31014-31028 (2014)
RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis
You H, et al.
Nucleic Acids Research, 45(1), 206-214 (2016)
Ruofan Cao et al.
Journal of biomedical optics, 25(1), 1-16 (2020-01-11)
Two-photon fluorescence lifetime imaging microscopy (FLIM) is widely used to capture autofluorescence signals from cellular components to investigate dynamic physiological changes in live cells and tissues. Among these intrinsic fluorophores, nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD)-essential
Atomic Structure of the Cystic Fibrosis Transmembrane Conductance Regulator
Zhang Z, et al.
Cell, 167(6), 1586-1597 (2016)
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