10148334001
Roche
2,3-Diphosphoglycerate (2,3-DPG)
Synonym(s):
2,3-dpg, glycerate-2,3-biphosphate, test combination
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352200
usage
sufficient for ~30 tests
manufacturer/tradename
Roche
shipped in
wet ice
storage temp.
2-8°C
General description
UV-test for the determination of 2,3-DPG in blood research samples.
Note: In addition, perchloric acid as auxiliary reagent is required.
Note: In addition, perchloric acid as auxiliary reagent is required.
Application
The test is used for the determination of 2,3-diphosphoglycerate in blood in the range of 0.02 to 0.15 μmol in life science research applications.
2,3-diphosphoglycerate (2,3-DPG) kit has been used to determine the levels of 2,3 DPG and ATP in fresh and stored human red blood cells.
2,3-diphosphoglycerate (2,3-DPG) kit has been used to determine the levels of 2,3 DPG and ATP in fresh and stored human red blood cells.
Biochem/physiol Actions
2,3-diphosphoglycerate (2,3-DPG) in red blood cells (RBC) determines the ease with which hemoglobin releases oxygen in the tissues. The higher the level of erythrocytic 2,3DPG, the lower is the affinity to oxygen and vice versa. pH levels during acid-base disorder influence oxygen affinity by affecting both 2,3-DPG metabolism and oxyhaemoglobin dissociation curve. Intracellular 2,3DPG concentrations are influenced by phosphate levels, and low erythrocyte levels of 2,3DPG are linked with hypophosphatemia.
Packaging
1 kit containing 5 components.
Preparation Note
Stabilizers: EDTA contained in the buffer solution of bottle 1 of the 2,3-Diphosphoglycerate Test Kit stabilizes enzymes when dissolved in this buffer.
Note: In addition, perchloric acid as auxiliary reagent is required.
Note: In addition, perchloric acid as auxiliary reagent is required.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Triethanolamine buffer, pH 7.6, with MgCl2 and EDTA
- ATP and NADH, 2 bottles
- PGM, PGK, GAP-DH, TIM and GDH, lyophilizate
- Phosphoglyceratemutase (PGM), lyophilizate
- Glycolate-2-phosphate, tricyclohexylammonium salt
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Eye Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
does not flashNot applicable
Flash Point(C)
does not flashNot applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service