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10223581001

Roche

Poly(dA)

Name: Poly(dA)
Brand: ROCHE

Poly-deoxy-adenylic acid

Synonym(s):

PCR

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About This Item

UNSPSC Code:

41105600

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Properties

form

lyophilized

mol wt

330 Da

packaging

pkg of 5 U

manufacturer/tradename

Roche

absorbance ratio

A_290/260 nm 0.03-0.12, A_280/260 nm 0.28-0.39, A_250/260 nm 0.84-0.94

storage temp.

−20°C (−15°C to −25°C)

Description

General description

Poly(dA) is a polymer of polydeoxyadenylic acid and takes up a single stranded structure. It is susceptible to UV irradiation resulting in photoreaction due to the presence of adjacent deoxyadenylic acid units. Poly(dA) along with β-glucan is used for the conjugation and delivery of DNA into cell lines.

Application

Poly(dA) is suitable for use in:

DNA polymerase assay and as a buffer component for the dilution of protein DNA complex for electrophoretic mobility shift assay (EMSA) studies
as ssDNA substrate for DNA helicase gene 40 product (G40P) NTP hydrolysis
in Pyrococcus abyssi DNA polymerase assay

Other Notes

1 A₂₆₀ unit corresponds to 40 μg ssDNA.

Chain Length Mean chain length lot 20-17: 250-500 bases.

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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A specific photoreaction in polydeoxyadenylic acid

Porschke D

Proceedings of the National Academy of Sciences of the USA, 70(9), 2683-2686 (1973)

Bacillus subtilis bacteriophage SPP1 hexameric DNA helicase, G 40 P, interacts with forked DNA

Ayora S, et al.

Nucleic Acids Research, 30(11), 2280-2289 (2002)

Poly(dA:dT), a ubiquitous promoter element that stimulates transcription via its intrinsic DNA structure.

V Iyer et al.

The EMBO journal, 14(11), 2570-2579 (1995-06-01)

Many yeast promoters contain homopolymeric dA:dT sequences that affect nucleosome formation in vitro and are required for wild-type levels of transcription in vivo. Here, we show that poly(dA:dT) is a novel promoter element whose function depends on its intrinsic structure

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Global Trade Item Number

SKU	GTIN
10223581001	04061838669346