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Merck
CN

11102982001

Roche

Mro I (Acc III)

from Micrococcus roseus

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About This Item

UNSPSC Code:
41105600

Key Documents

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biological source

bacterial (Micrococcus roseus)

form

solution

packaging

pkg of 100 U (1 - 5 U/μl)

manufacturer/tradename

Roche

parameter

37 °C optimum reaction temp.

shipped in

dry ice

storage temp.

−20°C

General description

Restiction endonuclease Mro I (Acc III) recognizes the sequence TCCGGA.

Biochem/physiol Actions

Recognition sites: T↓*CCGG°A
T*CCGG°A
Restriction site: T↓*CCGG°A
T↓*CCGG°A
Heat inactivation: There is no information available whether or not Mvn I can be heat inactivated.
Number of cleavage sites on different DNAs
  • λ: 24
  • φX174: 0
  • Ad2: 8
  • M13mp7: 0
  • pBR322: 1
  • pBR328: 1
  • pUC18: 0
  • SV40: 0

Analysis Note

SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity
  • A: 100%
  • B: 0-10%
  • H: 0-10%
  • L: 50-75%
  • M: 50-75%

Activity in PCR buffer: Not tested

Other Notes

For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity that completely cleaves 1 μg λDNA in one hour at +37 °C in a total volume of 25 μl (1x) SuRE/Cut Buffer A.

Kit Components Only

Product No.
Description
  • Enzyme Solution
  • SuRE/Cut Buffer A 10x concentrated

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
14140920
70381121
70294321
70291320
70215620

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K Inagaki et al.
Bioscience, biotechnology, and biochemistry, 57(10), 1716-1721 (1993-10-01)
A restriction endonuclease, Aor13HI, an isoschizomer of BspMII, was purified to homogeneity from cell extracts of Acidiphilium organovorum strain 13H. The enzyme has a molecular mass of 60,000 daltons and consists of two subunits identical in molecular mass of 30,000

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