11210238001
Roche
DIG Glycan Differentiation Kit
usage
sufficient for 25 blots (10 cm x 10 cm each)
manufacturer/tradename
Roche
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shipped in
wet ice
storage temp.
2-8°C
General description
Characterize carbohydrate moieties of glycoproteins or other, correspondingly fixed, glycoconjugates.
Application
The DIG Glycan Differentiation Kit is used to analyze carbohydrate structures by the specific binding of selected lectins. In addition to the differentiation of glycoproteins bound to nitrocellulose membranes, the lectins are also suited for the identification of carbohydrate structures on tissue sections.
Biochem/physiol Actions
Detection of digoxigenin-labeled lectins bound to specific carbohydrate structures on blots. Detection is performed using anti-digoxigenin alkaline-phosphatase conjugates. The digoxigenin-labeled lectins enable differentiation between complex and high-mannose chains, differentiation between a(2,3) and a(2,6) linkage of terminal sialic acids, and identification of the core disaccharide Galß(1,3)GalNAc of O-glycans.
Packaging
1 kit containing 13 components.
Analysis Note
Function tests: With the lectins GNA, SNA, MAA, and DSA 0.1 μg, and with the lectin PNA 1 μg of the control glycoproteins are detectable after a 1-min incubation in the staining solution.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Lectin GNA, digoxigenin-labeled
- Lectin SNA, digoxigenin-labeled
- Lectin MAA, digoxigenin-labeled
- Lectin PNA, digoxigenin-labeled
- Lectin DSA, digoxigenin-labeled
- Anti-digoxigenin-AP
- NBT/BCIP Solution
- Carboxypeptidase Y, Control Glycoprotein
- Transferrin, Control Glycoprotein, HIV tested
- Fetuin, Control Glycoprotein
- Asialofetuin, Control Glycoprotein
- Blocking Reagent
- Ponceau S solution
See All (13)
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 - Acute Tox. 4 - Acute Tox. 4 Oral - Aquatic Chronic 2 Inhalation - Resp. Sens. 1 Dermal - Skin Sens. 1
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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