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Merck
CN

11210238001

Roche

DIG Glycan Differentiation Kit

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About This Item

UNSPSC Code:
23201100
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usage

sufficient for 25 blots (10 cm x 10 cm each)

manufacturer/tradename

Roche

greener alternative product characteristics

Designing Safer Chemicals
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shipped in

wet ice

storage temp.

2-8°C

General description

Characterize carbohydrate moieties of glycoproteins or other, correspondingly fixed, glycoconjugates.

Application

The DIG Glycan Differentiation Kit is used to analyze carbohydrate structures by the specific binding of selected lectins. In addition to the differentiation of glycoproteins bound to nitrocellulose membranes, the lectins are also suited for the identification of carbohydrate structures on tissue sections.

Biochem/physiol Actions

Detection of digoxigenin-labeled lectins bound to specific carbohydrate structures on blots. Detection is performed using anti-digoxigenin alkaline-phosphatase conjugates. The digoxigenin-labeled lectins enable differentiation between complex and high-mannose chains, differentiation between a(2,3) and a(2,6) linkage of terminal sialic acids, and identification of the core disaccharide Gal(1,3β(1,3)GalNAc of O-glycans.

Packaging

1 kit containing 13 components.

Analysis Note

Function tests: With the lectins GNA, SNA, MAA, and DSA 0.1 μg, and with the lectin PNA 1 μg of the control glycoproteins are detectable after a 1-min incubation in the staining solution.

Other Notes

For life science research only. Not for use in diagnostic procedures.


Kit Components Only

Product No.
Description

  • Lectin GNA, digoxigenin-labeled

  • Lectin SNA, digoxigenin-labeled

  • Lectin MAA, digoxigenin-labeled

  • Lectin PNA, digoxigenin-labeled

  • Lectin DSA, digoxigenin-labeled

  • Anti-digoxigenin-AP

  • NBT/BCIP Solution

See All (13)

signalword

Danger

Hazard Classifications

Acute Tox. 3 - Acute Tox. 4 - Acute Tox. 4 Oral - Aquatic Chronic 2 Inhalation - Resp. Sens. 1 Dermal - Skin Sens. 1

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Regulatory Information

监管及禁止进口产品

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