Product Name
Anti-Digoxigenin, Fab fragments, from sheep
biological source
sheep
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
packaging
pkg of 1 mg
manufacturer/tradename
Roche
technique(s)
ELISA: 2-4 μg/mL
immunohistochemistry: 0.5-1 μg/mL
western blot: 0.5-2 μg/mL
isotype
IgG1
storage temp.
2-8°C
Analysis Note
Preparation: After immunization with digoxigenin, sheep IgGs are purified by ion exchange chromatography. Specific IgGs are isolated by immunosorption. Fab fragments obtained after papain digestion are purified by gel filtration and lyophilized.
Application
Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.
Use Anti-Digoxigenin, Fab fragments for the detection of digoxigenin-labeled compounds using:
Use Anti-Digoxigenin, Fab fragments for the detection of digoxigenin-labeled compounds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
- Flow cell construction.
Biochem/physiol Actions
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Features and Benefits
Specific to digoxigenin and digoxin.
General description
Anti-Digoxigenin, Fab fragments are Fab fragments from polyclonal anti-digoxigenin antibodies. Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems.
Other Notes
For life science research only. Not for use in diagnostic procedures.
The number of amino groups in the Fab fragments is not known, because polyclonal antibodies are not sequenced. But as a rule of thumb full length antibodies contain about 80 amino groups on average.
Preparation Note
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
Storage conditions (working solution): Diluted antibody is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!
- ELISA: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 1 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Storage conditions (working solution): Diluted antibody is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!
Add 1 ml PBS to a final concentration of 1 mg/ml. Centrifuge reconstituted antibody for 5 minutes at 10,000 rpm prior to each use, and pipet the necessary amount carefully from the surface.
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Regulatory Information
常规特殊物品
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