biological source
sheep
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
packaging
pkg of 200 μg
manufacturer/tradename
Roche
isotype
IgG
storage temp.
2-8°C
General description
Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.
Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life.
Application
Anti-Digoxigenin has been used in DNA tethering. It has been used to attach DNA molecule to the glass surface of the flow cell.
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
Biochem/physiol Actions
In the presence of Na+, Mg2+ and ATP, digoxigenin inhibits sodium pumps.
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Preparation Note
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
- ELISA: for coating: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
Add 1 ml PBS to a final concentration of 200 μg/ml.
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption.
Analysis Note
No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
常规特殊物品
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Methods in Molecular Biology (2011)
Affinity Labeling of a Sulfhydryl Group in the Cardiacglycoside Receptor Site of Na+/K+?ATPase by N?Hydroxysuccinimidyl Derivatives of Digoxigenin.
Antolovic R, et al.
European Journal of Biochemistry, 227(1?2), 61-67 (1995)
Jonathan Silver et al.
Biosensors & bioelectronics, 63, 117-123 (2014-07-30)
We describe a proof-of-principle, immune sandwich assay in which immune complexes link micron-size beads via DNA tethers to a sensor surface. The number of tethered beads, counted using low-magnification microscopy, provides a measure of the concentration of analyte. The prototype
Nonradioactive labeling of probe with digoxigenin by polymerase chain reaction.
Lion T and Haas O A
Analytical Biochemistry, 188(2), 335-337 (1990)
Pengyu Hao et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(30), 17775-17784 (2020-07-17)
DNA mismatch repair (MMR), the guardian of the genome, commences when MutS identifies a mismatch and recruits MutL to nick the error-containing strand, allowing excision and DNA resynthesis. Dominant MMR models posit that after mismatch recognition, ATP converts MutS to
Articles
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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