Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
12352207
NACRES:
NA.54
Product Name
Hybridoma Fusion and Cloning Supplement, solution, suitable for hybridoma, Mycoplasma, tested
description
HFCS
sterility
0.2 μm filtered
form
solution
packaging
pkg of 10 mL (50x)
manufacturer/tradename
Roche
technique(s)
cell culture | hybridoma: suitable
impurities
Mycoplasma, tested
10 EU/mL Endotoxin (LAL)
solubility
water: miscible
storage temp.
−20°C
Quality Level
Analysis Note
Biological activity: Assayed for high cloning efficiency of a hybridoma cell line.
Application
Hybridoma Fusion and Cloning Supplement (HFCS) is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. HFCS replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.
General description
Medium supplement composed of albumin, insulin, transferrin, cytokines, a cholesterol source, other defined organic and inorganic compounds and fetal calf serum (final concentration: 0.5%).
HFCS contains human proteins. The raw material from which the human proteins were isolated, has been tested for the presence of Hepatitis B surface Antigen (HBsAg) and HIV-1/2 antibodies and found to be negative (according to current quality control procedures).
HFCS contains human proteins. The raw material from which the human proteins were isolated, has been tested for the presence of Hepatitis B surface Antigen (HBsAg) and HIV-1/2 antibodies and found to be negative (according to current quality control procedures).
Other Notes
For life science research only. Not for use in diagnostic procedures.
Physical form
Solution (50x concentrated, pH 7.4); filtered through 0.2 μm pore size membrane
Preparation Note
Working concentration: HFCS (50x) is diluted 1:50 (v/v) with basal medium. It is strongly suggested that you use RPMI 1640. The final medium should also contain L-glutamine and sodium bicarbonate.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Regulatory Information
高风险级别生物产品--人源产品
动植物来源培养基
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Mepur H Ravindranath et al.
Blood, 121(11), 2013-2028 (2013-01-12)
The US Food and Drug Administration approved intravenous immunoglobulin (IVIg), extracted from the plasma of thousands of blood donors, for removing HLA antibodies (Abs) in highly sensitized patients awaiting organ transplants. Since the blood of healthy individuals has HLA Abs
Toshiyuki Sasaki et al.
International journal of cancer, 134(7), 1558-1570 (2013-10-10)
Phenotypic expression of human leukocyte antigen (HLA)-E on the surface of tumor lesions includes intact heterodimer [HLA-E heavy chain and β2-microglobulin (β2m)] and β2m-free monomer. Anti-HLA-E monoclonal antibodies (mAbs), MEM-E/02 or 3D12 bind to the peptide sequences in β2m-free HLA-E
Related Content
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service