11667475001
Roche
Anti-HA-Peroxidase
from mouse IgG2bκ
Synonym(s):
antibody
biological source
mouse
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
12CA5, monoclonal
Assay
≥95% (HPLC and SDS-PAGE)
form
solution
packaging
pkg of 50 μg (500 μl)
manufacturer/tradename
Roche
concentration
0.1 mg/mL
isotype
IgG2bκ
epitope sequence
YPYDVPDYA
storage temp.
−20°C
General description
Anti-HA-Peroxidase is a monoclonal antibody to the HA peptide, conjugated to peroxidase. Anti-HA-Peroxidase recognizes the HA nonapeptide sequence (YPYDVPDYA), derived from the human influenza virus hemagglutinin protein (amino acids 98-106). The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".
Immunogen
Amino acids 98-106 from the human influenza virus hemagglutinin protein
Application
- Use Anti-HA-Peroxidase for the immunochemical detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:
- Dot blot
- Western blot
Note: For experiments in which sensitivity is not critical, use Anti-HA-Peroxidase (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity.
Biochem/physiol Actions
Anti-HA-Peroxidase recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
The antibody reacts with the HA epitope, a nonapeptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein.
Preparation Note
Stabilizers: BSA, sucrose, 0.02% thymol
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
Working solution: 20-fold dilution of Western Blocking Reagent in 1x PBS.
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
- Western blot: 0.1 μg/ml
Working solution: 20-fold dilution of Western Blocking Reagent in 1x PBS.
Analysis Note
Each lot of Anti-HA antibody is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
常规特殊物品
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