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Roche

ABTS™ Solution

solution (ready-to-use), suitable for ELISA

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Synonym(s):
ABTS™ Solution, ABTS

form

solution (ready-to-use)

Quality Level

100

usage

sufficient for 1,500-3,000 assays (ELISA)

packaging

pkg of 3 × 100 mL

manufacturer/tradename

Roche

technique(s)

ELISA: suitable

storage temp.

2-8°C

Related Categories

General description

ABTS™ Solution is a ready to use substrate for peroxidase-driven indicator reactions. It contains 2,2-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and H2O2 in glycin/citric acid buffer and is available in slightly green color.

Application

ABTS Solution is a chromogenic substrate for peroxidase in ELISA assays.

Biochem/physiol Actions

2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS™ acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS™ is a highly sensitive, specific and reproducible technique This solution is an excellent substrate for enzyme immunoassays with horseradish peroxidase as a marker enzyme.

Physical form

Reaction product:
Color: green
Evaluation: photometric (405nm)

Analysis Note

Absorption: ABTS absorption spectrum reference must always be measured and is determined by a spectrum. The Reference wavelength of 490 nm or higher should be selected due to no measureable absorption. The Reference absorption is usually automatically subtracted by the Plate Reader (background).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ABTS is a trademark of Roche

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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Sylvia Herter et al.
Molecular cancer therapeutics, 12(10), 2031-2042 (2013-07-23)
We report the first preclinical in vitro and in vivo comparison of GA101 (obinutuzumab), a novel glycoengineered type II CD20 monoclonal antibody, with rituximab and ofatumumab, the two currently approved type I CD20 antibodies. The three antibodies were compared in
Yuanyuan Kuang et al.
EBioMedicine, 57, 102825-102825 (2020-06-20)
Numerous currently incurable human diseases have been causally linked to mutations in connexin (Cx) genes. In several instances, pathological mutations generate abnormally active Cx hemichannels, referred to also as "leaky" hemichannels. The goal of this study was to assay the
H Matsuda et al.
The Japanese journal of experimental medicine, 54(3), 131-138 (1984-06-01)
A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as a substrate for horseradish peroxidase (HRP) conjugate was studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for
Gang Chen et al.
Biomolecules, 11(10) (2021-10-24)
Most recently, a technology termed TRIM-Away has allowed acute and rapid destruction of endogenous target proteins in cultured cells using specific antibodies and endogenous/exogenous tripartite motif 21 (TRIM21). However, the relatively large size of the full-size mAbs (150 kDa) results
Monica Musiani et al.
Nature protocols, 2(10), 2502-2510 (2007-10-20)
PCR is an established technique providing rapid and highly productive amplification of specific DNA sequences. The demand for equally rapid, sensitive and objective methods to achieve detection of PCR products has led to the coupling of PCR with ELISA. PCR-ELISA

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