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Roche

NBT/BCIP Ready-to-Use Tablets

tablet, pkg of 20 tablets

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Synonym(s):
NBT/BCIP

form

tablet

mol wt

Mr 433.6 (BCIP toluidine salt)
Mr 817.7 (NBT)

packaging

pkg of 20 tablets

manufacturer/tradename

Roche

storage temp.

2-8°C

Related Categories

General description

BCIP is the alkaline phosphatase (AP) substrate, which after dephosphorylation is oxidized by NBT to yield a dark-blue indigo precipitating dye. NBT is thereby reduced to a dark-blue precipitating dye and serves to intensify the color reaction making the detection more sensitive.
The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Application

NBT/BCIP Ready-to-Use tablets has been used in:
  • in situ hybridization.
  • western blot
  • southern blot
  • northern blot
  • colony and plaque lifts
  • immunohistochemistry
  • immunocytochemistry
Note: NBT/BCIP may be used with both nitrocellulose and nylon membranes.

Preparation Note

Working solution: Preparation of Color Substrate Solution
Add 1 tablet to 10 ml double-dist. water to prepare a ready-to-use staining solution.
Composition of staining solution (pH 9.5) after addition of double-dist. water: 0.4 mg/ml NBT; 0.19 mg/ml BCIP; 100 mM Tris buffer, pH 9.5; 50 mM MgSO4
Information Note: The color of the solution can vary from slightly yellowish-violet to light brown.
Important Note: Prepare the solution shortly before use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(C)

does not flash

Certificates of Analysis (COA)

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Jifeng Bian et al.
PLoS pathogens, 17(7), e1009748-e1009748 (2021-07-27)
Prions are infectious proteins causing fatal, transmissible neurodegenerative diseases of animals and humans. Replication involves template-directed refolding of host encoded prion protein, PrPC, by its infectious conformation, PrPSc. Following its discovery in captive Colorado deer in 1967, uncontrollable contagious transmission
Loss of fractalkine signaling exacerbates axon transport dysfunction in a chronic model of glaucoma
Breen KT, et al.
Frontiers in Neuroscience, 10, 526-526 (2016)
Stefan Dhein, Friedrich Wilhelm Mohr, Mario Delmar
Practical Methods in Cardiovascular Research (2006)
Factors associated with seroconversion to the major piroplasm surface protein of the bovine haemoparasite Theileria orientalis
Jenkins C and Bogema DR
Parasites & vectors, 9(1), 106-106 (2016)
Cheryl Jenkins et al.
Parasites & vectors, 9, 106-106 (2016-02-26)
Bovine theileriosis caused by Theileria orientalis is an emerging disease of cattle in the Asia-Pacific region where it causes a significant economic burden to meat and milk production. While host immunological responses to the lymphocyte-transforming species of Theileria, T. parva
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Protocols

NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting

NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting

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