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About This Item
Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
PubChem Substance ID:
UNSPSC Code:
41116100
Beilstein/REAXYS Number:
505995
MDL number:
vapor density
1.55 (vs air)
vapor pressure
0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
assay
99%
form
liquid
autoignition temp.
932 °F
mol wt
45.04
packaging
pkg of 500 mL
manufacturer/tradename
Roche
technique(s)
hybridization: suitable
color
colorless
refractive index
n20/D 1.447 (lit.)
pH
4-10 (20 °C, 200 g/L)
bp
210 °C (lit.)
mp
2-3 °C (lit.)
density
1.134 g/mL at 25 °C (lit.)
absorption
≤0.08 at 290 nm
shipped in
wet ice
storage temp.
2-8°C
SMILES string
NC=O
InChI
1S/CH3NO/c2-1-3/h1H,(H2,2,3)
InChI key
ZHNUHDYFZUAESO-UHFFFAOYSA-N
General description
Formamide is an organic solvent, which allows for the denaturation and renaturation of nucleic acids at room temperature. This is particularly useful for protocols where reaction times are long and high temperatures would damage biological activity through chain scissions and depurination etc. Formamide reduces thermal stability of double stranded nucleic acids and is generally used for DNA renaturation or DNA-RNA hybridization. Specificity and rate of reaction are determined by formamide concentration and temperature of the reaction.
Application
Formamide has been used in:
in situ hybridization
in situ hybridization
- as a component of 2X denaturing loading dye for RNA polyacrylamide gel electrophoresis
- for the extraction of evans blue dye in hindlimb of mice in vascular leakage assay
- as a component of stop buffer used in cleavage and polyadenylation activity assays
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Analysis Note
Contaminants: ≤5 ppm heavy metals (as Pb), ≤5 ppm Fe
Other Notes
For life science research only. Not for use in diagnostic procedures.
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signalword
Danger
hcodes
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
305.6 °F
flash_point_c
152 °C
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Nucleic acid reassociation in formamide.
B L McConaughy et al.
Biochemistry, 8(8), 3289-3295 (1969-08-01)
mRNA deadenylation is coupled to translation rates by the differential activities of Ccr4-Not nucleases
Webster MW, et al.
Molecular Cell, 70(6), 1089-1100 (2018)
Fluorescence in situ hybridization (FISH) protocol in human sperm
Sarrate Z and Anton E
Journal of Visualized Experiments, (31), e1405-e1405 (2009)