biological source
rat
Quality Level
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
clone 3F10, monoclonal
form
lyophilized (clear, colorless solution after reconstitution)
packaging
pkg of 25 U (25 μg)
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
storage temp.
2-8°C
Related Categories
General description
Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase.
The Anti-HA High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5, which was originally used to study how the immune system recognizes the influenza hemagglutinin protein, a surface glycoprotein required for infectivity of the human virus. However, the principal use of the Anti-HA antibody is the detection and purification of proteins whose encoding DNA sequences have been fused to the HA epitope sequence by recombinant techniques, that is epitope tagging. The ability to prepare such epitope-tagged proteins and locate them with the Anti-HA antibody in subsequent experiments has enabled researchers to determine:
- The size, cellular localization, and abundance of proteins produced by newly discovered genes.
- Post-translational modifications of proteins.
- The movement of proteins within cell membranes.
- The identity of proteins within functional protein complexes.
- The function of proteins that are unstable, difficult to purify, or share epitopes with a number of other proteins.
Immunogen
The epitope consists of amino acids 98-106 from the human influenza virus hemagglutinin protein.
Application
- Use Anti-HA-Peroxidase, High Affinity for the detection of HA-tagged recombinant proteins using: ELISA
- Western blot
Biochem/physiol Actions
Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein.
This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
Preparation Note
Stabilizers: proteinaceous stabilizers
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
- Dot blot: 50 mU/ml
- ELISA: 25 mU/ml
- Western blot: 50 mU/ml
Add 1.0 ml double-distilled water for a final concentration of 25 U/mL.
Rehydrate for 10 min prior to use.
Rehydrate for 10 min prior to use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
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