recombinant
expressed in E. coli (AP401 (k))
form
solution
usage
sufficient for 100 reactions, sufficient for 20 reactions
packaging
pkg of 1,000 U (11785826001 [20 μl]), pkg of 5,000 U (11785834001 [100 μl])
manufacturer/tradename
Roche
parameter
42-43 °C optimum reaction temp.
technique(s)
RT-PCR: suitable, RT-qPCR: suitable
input
purified RNA
detection method
probe-based
storage temp.
−20°C
General description
Expand™ Reverse Transcriptase is a RNA directed DNA polymerase optimized for amplification of cDNA fragments up to 13.5 kb in a two-step RT-PCR assay. The enzyme is a genetically engineered version of the Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV-RT). A point mutation within the RNase H sequence reduces RNase H activity to undetectable levels. Detectable improvements are obtained with respect to higher amounts of full-length cDNA transcripts and longer transcripts compared to using the native M-MuLV-RT.
Application
Expand™ Reverse Transcriptase has been used in real-time PCR analysis for conversion of isolated RNA into cDNA. It has also been used in reverse transcription (RT). Expand™ Reverse Transcriptase may also be used for analyzing the sequence of the cDNA and comparing it to the genomic sequence. Important information can be obtained on intervening sequences (introns) and genomic recombinations.
Biochem/physiol Actions
Heat inactivation: 95 °C for 2 min
Reverse Transcriptase enzyme for RT-PCR reactions up to 3 kb
Reverse Transcriptase enzyme for RT-PCR reactions up to 3 kb
Features and Benefits
- Obtain higher amounts of full-length cDNA transcripts and longer transcripts up to 13.5kb
- No RNase H activity
- Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
- Ideal for two-Step RT-PCR assay
- Purification of cDNA before PCR reaction is not necessary
Packaging
1 set containing 3 components
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity that incorporates 1.0 nmol TMP into acid insoluble products in 10 min at +37 °C with poly(A)+ × (dT)15 as substrate.
Volume Activity: 50 U/μl
Volume Activity: 50 U/μl
Legal Information
Expand is a trademark of Roche
Kit Components Only
Product No.
Description
- Expand Reverse Transcriptase 50 U/μl
- Expand Reverse Transcriptase Buffer 5x concentrated
- DTT Stock Solution 100 mM
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_c
does not flash
Regulatory Information
常规特殊物品
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M Hatzfeld et al.
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We have developed a duplex real-time RT-PCR assay for profiling antiviral inhibitors of four dengue virus (DENV) serotypes. In this assay, the primers and the probe for amplifying DENV were designed in the conserved regions of the genome after aligned
André Spychala et al.
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Initially, the function of the fat mass and obesity associated (Fto) gene seemed to be primarily the regulation of the body weight. Here we show that loss of Fto results in a hyperactivation of the hypothalamic-pituitary-adrenal (HPA) axis. In consequence
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