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TAQKB

Roche

KAPA Taq PCR Kit

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Synonym(s):
PCR, Taq
NACRES:
NA.54

shelf life

≤18 mo.

Quality Level

100

feature

dNTPs included: no
hotstart: no

packaging

pkg of 250 U (KK1014)
pkg of 2500 U (BK1000)
pkg of 500 U (KK1015)
pkg of 5000 U (BK1002)

manufacturer/tradename

Roche

technique(s)

PCR: suitable

input

purified DNA

storage temp.

−20°C

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Application

KAPA Taq PCR Kit has been used in:
  • High throughput PCR
  • Amplification of low copy DNA templates
  • Multiplex PCR
  • Specific amplification of complex templates
  • RT-PCR
  • random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)
  • Polymerase chain reaction (PCR)
  • Genotyping

Biochem/physiol Actions

KAPA Taq PCR Kit, which contains KAPA Taq DNA Polymerase, is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart® DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

Features and Benefits

High performance:
  • Improved sensitivity, specificity, and yields
  • Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product

Quick Notes:
  • KAPA Taq DNA Polymerase can replace any commercial Taq DNA polymerase in an existing protocol.
  • The final MgCl2 concentration may need to be optimized to account for differences in buffer formulation.
  • KAPA Taq Buffers contain MgCl2 at a final concentration of 1.5 mM. Buffer A is recommended as first approach and for applications requiring high yields. Buffer B is recommended for applications where high sensitivity is required (e.g. when the template is limiting). Both buffers may be evaluated to determine the buffer most suitable for a specific application.
  • The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

Quality

Each batch of KAPA Taq DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230Assay). KAPA Taq Ready Mixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C forshort-term use (up to 1 month). Return to -20°C for long term storage.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Kit Components Only

Product No.
Description
  • KAPA Taq Standard or HotStart® DNA Polymerase 5 U/μL
  • 10X KAPA Taq Buffer A
  • 10X KAPA Taq Buffer B
  • MgCl2 25 mM

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

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Direct conversion of mouse embryonic fibroblasts into functional keratinocytes through transient expression of pluripotency-related genes.
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