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About This Item
NACRES:
NA.54
UNSPSC Code:
41106300
shelf life
≤18 mo.
feature
dNTPs included: no, hotstart: no
packaging
pkg of 250 U (KK1014), pkg of 2500 U (BK1000), pkg of 500 U (KK1015), pkg of 5000 U (BK1002)
manufacturer/tradename
Roche
technique(s)
PCR: suitable, whole genome amplification: suitable
input
purified DNA
storage temp.
−20°C
Application
Biochem/physiol Actions
KAPA Taq PCR Kit, which contains KAPA Taq DNA Polymerase, is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart® DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.
Features and Benefits
High performance:
Quick Notes:
- Improved sensitivity, specificity, and yields
- Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product
Quick Notes:
- KAPA Taq DNA Polymerase can replace any commercial Taq DNA polymerase in an existing protocol.
- The final MgCl2 concentration may need to be optimized to account for differences in buffer formulation.
- KAPA Taq Buffers contain MgCl2 at a final concentration of 1.5 mM. Buffer A is recommended as first approach and for applications requiring high yields. Buffer B is recommended for applications where high sensitivity is required (e.g. when the template is limiting). Both buffers may be evaluated to determine the buffer most suitable for a specific application.
- The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C forshort-term use (up to 1 month). Return to -20°C for long term storage.
Analysis Note
Each batch of KAPA Taq DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230Assay). KAPA Taq Ready Mixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
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Kit Components Only
Product No.
Description
- KAPA Taq Standard or HotStart® DNA Polymerase 5 U/μL
- 10X KAPA Taq Buffer A
- 10X KAPA Taq Buffer B
- MgCl2 25 mM
Storage Class
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
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