Product Name
Taq DNA Polymerase (1 U/μl), dNTPack, suitable for PCR, optimum pH ~9.0 (20 °C)
usage
sufficient for ≤2,000 reactions (04738241001)
sufficient for ≤500 reactions (04738225001)
feature
dNTPs included
hotstart: no
packaging
pkg of 1,000 U (04738241001 [4 x 250 U])
pkg of 250 U (04738225001)
manufacturer/tradename
Roche
parameter
72 °C optimum reaction temp.
technique(s)
PCR: suitable
input
purified DNA
optimum pH
~9.0 (20 °C)
storage temp.
−20°C
Application
Taq DNA Polymerase (1 U/μl), dNTPack may be used for:
- PCR
- RT-PCR
- Other primer-extension reactions, such as sequencing and labeling
General description
Taq DNA Polymerase (1 U/μl), dNTPack, comprises Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. Taq DNA polymerase is a highly processive 5′-3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It is stable during prolonged incubations at elevated temperatures (+95 °C). Additionally, it exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. It accepts dNTP analogs as substrates. There is no difference in stability or performance when compared to the standard concentration of Taq DNA Polymerase.
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 1 U/μl
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 1 U/μl
Packaging
1 kit containing 5 components
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Regulatory Information
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Find documentation for the products that you have recently purchased in the Document Library.
Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase.
Y H Zhou et al.
Nucleic acids research, 19(21), 6052-6052 (1991-11-11)
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