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Merck
CN

04558

Calcein AM solution

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About This Item

Empirical Formula (Hill Notation):
C46H46N2O23
CAS Number:
148504-34-1
Molecular Weight:
994.86
EC Number:
200-664-3
UNSPSC Code:
12171500
MDL number:
MFCD05861516

Key Documents

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InChI key

XKFSBWQWNMZWFA-UHFFFAOYSA-N

InChI

1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45

concentration

1 mM in DMSO

refractive index

n20/D 1.479

fluorescence

λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+

suitability

suitable for fluorescence

shipped in

dry ice

storage temp.

−20°C

Biochem/physiol Actions

Fluorescent cell permeable derivative of calcein.

Other Notes

Cell membrane permeable reagent for staining viable cells

Regulatory Information

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E Giacomello et al.
BioTechniques, 26(4), 758-762 (1999-05-27)
Cell adhesion assays are widely used to identify novel cellular ligands, novel cell surface receptors for these ligands and to elucidate the mechanisms responsible for the underlying cellular and molecular interactions. We report here the development of a novel centrifugal
S A Weston et al.
Journal of immunological methods, 133(1), 87-97 (1990-10-04)
16 fluorochromes were examined for their ability to label viable lymphocytes in vitro and yield fluorescence detectable by fluorescence microscopy and flow cytometry. Of these fluorochromes, four intracellular dyes were found to be suitable for in vivo migration studies. They

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