05475
Bovine Serum Albumin
fraction V, ≥96% (GE)
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
assay
≥90% protein basis, ≥96% (GE)
mol wt
Mr ~67000
loss
≤5% loss on drying
solubility
H2O: 40 mg/mL, clear to faintly turbid, faintly yellow to greenish-yellow
storage temp.
2-8°C
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Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
by a modified Cohn procedure, using cold ethanol, pH and low temperature precipitation.
Other Notes
Sales restrictions may apply
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Preparation and properties of serum and plasma proteins; a system for the separation into fractions of the protein and lipoprotein components of biological tissues and fluids.
E J COHN et al.
Journal of the American Chemical Society, 68, 459-475 (1946-03-01)
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