05491
Albumin, Acetylated from bovine serum
BioReagent, lyophilized powder, ≥90.0% (GE)
Synonym(s):
Ac-BSA, Acetyl BSA, Acetylated BSA
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About This Item
biological source
bovine
grade
Molecular Biology
product line
BioReagent
assay
≥90.0% (GE)
form
lyophilized powder
impurities
HIV I and HIVII, HCV and HBsAg, tested negative
solubility
H2O: slightly soluble
foreign activity
DNase, RNase, protease, and alkaline phosphatase, none detected
storage temp.
2-8°C
Application
Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.
Albumin from bovine serum has been used for electron and immunoelectron microscopy. It has also been used for isolating and culturing neonatal rat cardiac ventricular myocytes.
Biochem/physiol Actions
Bovine serum albumin also referred to as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also trigger insulin dependent diabetes mellitus in humans.
Preparation Note
Albumin from bovine serum is slightly soluble in water.
from BSA by acetylation in order to inactivate nucleases.
Other Notes
In the preparation of a bromouridine conjugate in EIA using a monoclonal antibody against 5-bromo-2-deoxyuridine for the assessment of lymphoid cell proliferation
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Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Enzyme immunoassay using a monoclonal antibody against 5-bromo-2-deoxyuridine for the assessment of lymphoid cell proliferation.
T Ternynck et al.
Methods in enzymology, 150, 117-129 (1987-01-01)
J D Marsh et al.
Circulation, 98(3), 256-261 (1998-08-11)
The role of androgens in producing cardiac hypertrophy by direct action on cardiac myocytes is uncertain. Accordingly, we tested the hypothesis that cardiac myocytes in adult men and women express an androgen receptor gene and that myocytes respond to androgens
Giovanna Andreola et al.
The Journal of experimental medicine, 195(10), 1303-1316 (2002-05-22)
The hypothesis that FasL expression by tumor cells may impair the in vivo efficacy of antitumor immune responses, through a mechanism known as 'Fas tumor counterattack,' has been recently questioned, becoming the object of an intense debate based on conflicting
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 347558-5G | 04061826761687 |