06212
Esculin hydrate
analytical standard
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About This Item
Empirical Formula (Hill Notation):
C15H16O9 · xH2O
CAS Number:
Molecular Weight:
340.28 (anhydrous basis)
NACRES:
NA.24
PubChem Substance ID:
UNSPSC Code:
85151701
EC Number:
207-646-4
MDL number:
grade
analytical standard
Quality Level
assay
≥98.0% (HPLC)
optical activity
[α]/D -40.5±2.5°, c = 5% in pyridine
shelf life
limited shelf life, expiry date on the label
impurities
≤11% water
application(s)
food and beverages
format
neat
InChI
1S/C15H16O9.H2O/c16-5-10-12(19)13(20)14(21)15(24-10)23-9-3-6-1-2-11(18)22-8(6)4-7(9)17;/h1-4,10,12-17,19-21H,5H2;1H2/t10-,12-,13+,14-,15-;/m1./s1
InChI key
CQYPGSKIFJFVDQ-QWFKVUSTSA-N
Application
Esculin hydrate may be used as an analytical standard for:
- The detection of glucosidase inhibitors by thin-layer chromatography (TLC) bioautographic method.
- Library screening in the characterization of novel cellulase genes from uncultured microorganisms.
- Evaluation of mutagenic and antimutagenic activities of oligorutin and oligoesculin.
- Detecting β-glucosidase activity in the identification of novel cellulase genes from uncultured microorganisms in rabbit cecum.
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Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Yi Feng et al.
Applied microbiology and biotechnology, 75(2), 319-328 (2007-01-12)
A metagenomic cosmid library was prepared in Escherichia coli from DNA extracted from the contents of rabbit cecum and screened for cellulase activities. Eleven independent clones expressing cellulase activities (four endo-beta-1,4-glucanases and seven beta-glucosidases) were isolated. Subcloning and sequencing analysis
Fengchao Wang et al.
Microbiological research, 164(6), 650-657 (2009-02-24)
Four different environmental DNA libraries were prepared from microbial consortium collected from forest soil, dung of elephant, cow rumen and rotted tree. Seven independent clones specifying cellulase activities (five endo-beta-1,4-glucanases and two beta-glucosidases) were isolated and identified. Sequence analysis of
Mario O Salazar et al.
Phytochemical analysis : PCA, 18(3), 209-212 (2007-05-16)
A new bioautographic assay suitable for the localisation of beta-glucosidase inhibitors present in a complex matrix is described. Enzyme activity was detected using esculin as the substrate to produce esculetin, which reacts with ferric ion to form a brown complex.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 1030980005 | 04022536039923 |
| 1030980025 | 04022536039930 |
| 798533-100G | 04061832973647 |
| 798533-500G | 04061832973654 |
| D216305-1KG | 04061833561799 |
| D216305-10G | 04061833561782 |
| D216305-500G | 04061832997858 |
| V900145-25G | 04061833001202 |
| 798533-1KG | 04061824805253 |
| 798533-50G | 04061832973661 |
| D216305-12KG | 04061833639511 |
| D216305-25G | 04061833564608 |
| D216305-100G | 04061833561775 |
| D216305-2.5G | 04061833561805 |
| 06212-100MG | 04061838645937 |