07980
5-Aminofluorescein
p.a., ≥90% (HPLC)
Synonym(s):
Fluoresceinamine isomer I
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About This Item
Empirical Formula (Hill Notation):
C20H13NO5
CAS Number:
Molecular Weight:
347.32
UNSPSC Code:
12171500
EC Number:
222-043-6
Beilstein/REAXYS Number:
48395
MDL number:
grade
p.a.
assay
≥90% (HPLC)
impurities
≤1% 6-isomer (HPLC)
suitability
suitable for fluorescence
SMILES string
Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
Application
The two isomers are produced by the usual synthesis. Most publications imply they are interchangeable when used for the preparation of the isothiocyanate for fluorescent antibody work. In fact, most commercial preparations are not even labeled as to whether or not they are mixtures.
Other Notes
All known glycosaminoglycuronans react with this fluorescein derivative to yield fluorescent derivatives
Note: Do not confuse with fluorescamine.
Regulatory Information
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A Ogamo et al.
Carbohydrate research, 105(1), 69-85 (1982-07-01)
The uronic acid residues of all known glycosaminoglycuronans reacted with 5-aminofluorescein to yield fluorescent glycosaminoglycuronan derivatives, which showed fluorescence characteristics identical to those of fluorescein or 5-acetamidofluorescein. The fluorescent products could be purified by chromatography on Octyl-Sepharose; three preparations of
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