11367
Avidin, deglycosylated from hen egg white
≥10 U/mg
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About This Item
UNSPSC Code:
12352200
MDL number:
specific activity
≥10 U/mg
storage temp.
2-8°C
Analysis Note
may contain non-deglycosylated avidin
Other Notes
1 U corresponds to the amount of protein which binds 1 μg (+)-biotin at pH 7.5
Deglycosylation reduces non-specific binding as experienced with egg-white avidin, cf. streptavidin which lacks the glycoprotein component. Oligosaccharide side chain not required for ligand association
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Y Hiller et al.
The Biochemical journal, 248(1), 167-171 (1987-11-15)
A commercially available, purified preparation of avidin was found to comprise two polypeptide bands (Mr 18,000 and Mr 15,500 respectively). Both bands bound biotin as assessed by biotin overlays of protein blots. The Mr 15,500 polypeptide was found to differ
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