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Merck
CN

11945

Benedict′s reagent

for the qualitative determination of sugars

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About This Item

CAS Number:
UNSPSC Code:
12164500
NACRES:
NA.21
MDL number:
Technical Service
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Quality Level

technique(s)

photometry: suitable

impurities

cupric sulfate

application(s)

food and beverages
general analytical

SMILES string

[Cu+2].[Na+].[Na+].[S](=O)(=O)(O)O.[O-]C(=O)C(O)(CC(=O)[O-])CC(=O)[O-].[O-]C(=O)O.O

InChI

1S/C6H8O7.CH2O3.Cu.2Na.H2O4S.H2O/c7-3(8)1-6(13,5(11)12)2-4(9)10;2-1(3)4;;;;1-5(2,3)4;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);(H2,2,3,4);;;;(H2,1,2,3,4);1H2/q;;+2;2*+1;;/p-4

InChI key

QFKWSRIUZIYLCK-UHFFFAOYSA-J

General description

Benedict′s reagents comprise of copper sulphate, sodium carbonate which functions as a mild alkali, and sodium citrate. It is quite stable. On boiling this reagent with glucose, it gives a white precipitate of cuprous thiocyanate. It is primarily used for the estimation of glucose in urine.

Application

Benedict′s reagent may be used during TLC analysis of mesocotyl methanol extracts during a study to evaluate sorghum resistance associated with accumulation of undetected toxic host metabolites. It may also be also be used to synthesize an SEM image of the cubic shaped Cu2O with smooth surface.

Other Notes

Reagent used for the detection of reducing sugars and other reducing substances; detection limit: 0.01 % glucose in water


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pictograms

Exclamation markEnvironment

signalword

Warning

hcodes

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves



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R.L. Shriner, et al.
The Systematic Identification of Organic Compounds (1980)
Textbook of Medical Biochemistry.
Ramakrishnan S
Science, 18-19 (2004)
[Caustic lesions caused by test tablets used in semiquantitative urinary sugar determination (Clinitest)].
I Schlatter-Lanz
Schweizerische Rundschau fur Medizin Praxis = Revue suisse de medecine Praxis, 74(50), 1402-1406 (1985-12-10)



Global Trade Item Number

SKUGTIN
11945-10ML04061837900754
11945-1L04061838708670
11945-250ML04061838708724