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Merck
CN

14339

Bicinchoninic acid disodium salt hydrate

≥99.0% (HPLC)

Synonym(s):

2,2′-Biquinoline-4,4′-dicarboxylic acid disodium salt, 4,4′-Dicarboxy-2,2′-biquinoline disodium salt, BCA, Disodium 2,2′-biquinoline-4,4′-dicarboxylate

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About This Item

Empirical Formula (Hill Notation):
C20H10N2Na2O4 · xH2O
CAS Number:
979-88-4
Molecular Weight:
388.28 (anhydrous basis)
UNSPSC Code:
12171500
PubChem Substance ID:
329750454
MDL number:
MFCD10567219

Key Documents

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InChI

1S/C20H12N2O4.2Na.H2O/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;;/h1-10H,(H,23,24)(H,25,26);;;1H2/q;2*+1;/p-2

SMILES string

O.[Na+].[Na+].[O-]C(=O)c1cc(nc2ccccc12)-c3cc(C([O-])=O)c4ccccc4n3

InChI key

FAFVGCAYKZKOPT-UHFFFAOYSA-L

assay

≥99.0% (HPLC)

impurities

~12% water

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Other Notes

Photometric reagent for protein assay. Forms an intense purple complex with the cuprous ion and hence the basis of the analytical monitoring of cuprous ion produced in the biuret reaction. Demonstrates greater tolerance to interference from non-ionic detergents and simple buffer salts

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

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M G Redinbaugh et al.
Analytical biochemistry, 153(2), 267-271 (1986-03-01)
The bincinchoninic acid protein assay has been adapted for use with microtiter plates and a plate reader to reduce the time needed for analyses. The total volume of the assay was reduced to 0.21 ml with various ratios of sample
Martha Lappas
PloS one, 9(12), e115854-e115854 (2014-12-30)
Infection and inflammation, through their ability to increase pro-inflammatory cytokines and chemokines and adhesion molecules, are thought to play a central role in the pathophysiology of insulin resistance and type 2 diabetes. Recent studies have shown that glycogen synthase kinase
P K Smith et al.
Analytical biochemistry, 150(1), 76-85 (1985-10-01)
Bicinchoninic acid, sodium salt, is a stable, water-soluble compound capable of forming an intense purple complex with cuprous ion (Cu1+) in an alkaline environment. This reagent forms the basis of an analytical method capable of monitoring cuprous ion produced in
Elena Magrini et al.
The Journal of clinical investigation, 124(10), 4335-4350 (2014-08-27)
While tumor blood vessels share many characteristics with normal vasculature, they also exhibit morphological and functional aberrancies. For example, the neural adhesion molecule L1, which mediates neurite outgrowth, fasciculation, and pathfinding, is expressed on tumor vasculature. Here, using an orthotopic
Seray Adams et al.
PloS one, 9(11), e112945-e112945 (2014-11-22)
The kynurenine pathway (KP) is the principal route of L-tryptophan (TRP) catabolism leading to the production of kynurenine (KYN), the neuroprotectants, kynurenic acid (KYNA) and picolinic acid (PIC), the excitotoxin, quinolinic acid (QUIN) and the essential pyridine nucleotide, nicotinamide adenine
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