14402
Biotin, immobilized on Dextran
≥99.0% (TLC)
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About This Item
NACRES:
NA.25
UNSPSC Code:
12352200
biological source
synthetic
assay
≥99.0% (TLC)
form
powder
mol wt
Mr ~70000
extent of labeling
5-25 mol/mol substitution (biotin)
color
colorless to white
solubility
H2O: 50 mg/mL, slightly hazy, faintly yellow
application(s)
detection
storage temp.
2-8°C
Quality Level
General description
Biotin/vitamin B7/vitamin H is a water-soluble B complex vitamin. It consists of an uredo ring fused by a tetrahydrothiophene ring. Biotin is readily available in several foods, like whole grains, nuts, legumes, unpolished rice and egg yolk. The normal gut flora also produces this vitamin.
Application
Biotin, immobilized on dextran has been used in in vivo labeling of the lectin structures of vascular endothelium to demonstrate the functionality of CD31+ vessels.
Dextrans are used in many applications as volume extenders, stabilizers, matrix components, binding platforms, lubricants and physical structure components. Biotinylated dextran may be used to capture or detect avidin/strepavidin conjugated molecules.
Biochem/physiol Actions
Biotin acts as an important cofactor for carboxylase enzymes in various metabolic pathways. It is implicated in keratin synthesis and hence is used as a supplement for skin, hair and nail growth. Biotin functions as a coenzyme in the metabolism of fats, fatty acids and amino acids such as isoleucine and valine. It is involved in gluconeogenesis and citric acid cycle. Biotin is essential for cell growth.It assists the transfer of carbon dioxide and also maintains a steady blood sugar level.
Storage Class
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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N Rajakumar et al.
Brain research, 607(1-2), 47-53 (1993-04-02)
A powerful and versatile axonal tracing method using biotinylated dextran, a novel analogue of biotin, is described. Pressure injection of varying volumes of 5% biotinylated dextran into various parts of the brain and spinal cord resulted in Golgi-like retrograde labeling
Ying-Jen Chen et al.
Small (Weinheim an der Bergstrasse, Germany), 14(22), e1703705-e1703705 (2018-05-03)
The noninvasive monitoring of protein secretion of cells responding to drug treatment is an effective and essential tool in latest drug development and for cytotoxicity assays. In this work, a surface functionalization method is demonstrated for specific detection of protein
N Emans et al.
Biophysical journal, 69(2), 716-728 (1995-08-01)
A fluorescence assay of in vivo endosome fusion was developed and applied to define the kinetics of endosome fusion in baby hamster kidney (BHK) fibroblasts. The assay is based on an approximately 10-fold enhancement of the green fluorescence of BODIPY-avidin
Revascularization of the graft in obliterative bronchiolitis after heterotopic tracheal transplantation
Nemska S, et al.
Physiological Reports, 4(4) (2016)
A Review of the Use of Biotin for Hair Loss
Patel DP, et al.
Skin Appendage Disorder, 3(3), 166-166 (2017)
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