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Merck
CN

14728

Sigma-Aldrich

Calcein

≥93% (HPCE)

Synonym(s):

Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon

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About This Item

Empirical Formula (Hill Notation):
C30H26N2O13
CAS Number:
Molecular Weight:
622.53
Beilstein:
8181068
EC Number:
UNSPSC Code:
12171500
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Assay

≥93% (HPCE)

technique(s)

titration: suitable

solubility

H2O: soluble

fluorescence

λex 470 nm; λem 509 nm in PBS

suitability

suitable for fluorescence

SMILES string

OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O

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General description

Highly purified and consists of one isomer (the 4′,5′-isomer).

Application

Indicator for the fluorimetric determination of calcium (in presence of Mg) by complexometric titration; Determination of Ca in biological systems.

Regulatory Information

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Investigation of the use of calcein in the ultramicro fluorometric determination of calcium in serum.
J F Bandrowski et al.
Clinical chemistry, 18(11), 1411-1414 (1972-11-01)
V C Chiu et al.
Biophysical journal, 18(1), 3-22 (1977-04-01)
The fluorescent calcium indicator, calcein, has been used as a high-affinity indicator of Ca2+ in the aqueous phase at physiological pH in the study of high-affinity calcium binding to sarcoplasmic reticulum (SR). The binding constant of the indicator at physiological
T.Y. Toribara et al.
Talanta, 7, 248-248 (1961)
Wallach, D.F.H., et al.
Analytical Chemistry, 31, 456-456 (1959)

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