14728
Calcein
≥93% (HPCE)
Synonym(s):
Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon
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About This Item
Empirical Formula (Hill Notation):
C30H26N2O13
CAS Number:
Molecular Weight:
622.53
UNSPSC Code:
12171500
EC Number:
215-957-1
Beilstein/REAXYS Number:
8181068
SMILES string
OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O
assay
≥93% (HPCE)
technique(s)
titration: suitable
solubility
H2O: soluble
fluorescence
λex 470 nm; λem 509 nm in PBS
suitability
suitable for fluorescence
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General description
Highly purified and consists of one isomer (the 4′,5′-isomer).
Application
Indicator for the fluorimetric determination of calcium (in presence of Mg) by complexometric titration; Determination of Ca in biological systems.
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Investigation of the use of calcein in the ultramicro fluorometric determination of calcium in serum.
J F Bandrowski et al.
Clinical chemistry, 18(11), 1411-1414 (1972-11-01)
V C Chiu et al.
Biophysical journal, 18(1), 3-22 (1977-04-01)
The fluorescent calcium indicator, calcein, has been used as a high-affinity indicator of Ca2+ in the aqueous phase at physiological pH in the study of high-affinity calcium binding to sarcoplasmic reticulum (SR). The binding constant of the indicator at physiological
Wallach, D.F.H., et al.
Analytical Chemistry, 31, 456-456 (1959)
T.Y. Toribara et al.
Talanta, 7, 248-248 (1961)
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