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Merck
CN

17170

AEA Sporulation Broth (Base), modified

NutriSelect® Plus, according to APHA, powder, for Clostridium perfringens, pack of 500 g, suitable for microbiology

Synonym(s):

Clostridium sporulation broth

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Plus
Agency:
according to APHA
Form:
powder
Suitability:
Clostridium perfringens
Application(s):
food and beverages, microbiology

Key Documents

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Quality Level

200

agency

according to APHA

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

ammonium acetate, 1.5 g/L , biopeptone, 10 g/L , disodium phosphate, 4.36 g/L , magnesium sulfate.7H2O, 0.2 g/L , monopotassium phosphate, 0.25 g/L , yeast extract, 10 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.8±0.2 (25 °C)

application(s)

food and beverages, microbiology

suitability

Clostridium perfringens

Related Categories

Application

For early sporulation of Clostridium perfringens from foods.

Preparation Note

Suspend 26.31 g in 1 litre distilled water. Dispense the medium in 15 ml amounts in screw capped tubes and sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and add 0.6 ml of filter sterilized 10% raffinose and 0.2 ml each of sterile 0.66 M sodium carbonate and 0.32% cobalt chloride dropwise to each 15 ml base medium in the tubes. Just before use, steam the medium for 10 minutes and after cooling, add 0.2 ml of filter sterilized (freshly prepared) 1.5% sodium ascorbate to each tube of the medium.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP0632
BCCM6861
BCCM1239
BCCL5437
BCCM1009

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Joyshree Ganguly et al.
Anaerobe, 63, 102208-102208 (2020-05-11)
Single-cell analysis of microbial population heterogeneity is a fast growing research area in microbiology due to its potential to identify and quantify the impact of subpopulations on microbial performance in, for example, industrial biotechnology, environmental biology, and pathogenesis. Although several

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产气荚膜梭菌会导致未煮熟或未正确消毒的罐头食品和水受到污染。了解如何检测、鉴定和区分这种病原体。

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