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Merck
CN

18502

Oxidase Reagent according to Gordon-McLeod

suitable for microbiology

Synonym(s):

Gordon-McLeod Reagent

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About This Item

UNSPSC Code:
41171621
NACRES:
NA.85

Key Documents

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Agency

according to ISO 9308-1:2014

Quality Level

200

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

N,N-dimethyl-p-phenylenediamine hydrochloride, 0.15 g
water, 10 mL

packaging

pkg of 100 mL

storage condition

(Tightly closed)

technique(s)

microbe id | specific enzyme detection: suitable

antibiotic activity spectrum

Gram-negative bacteria

application(s)

clinical testing
environmental
food and beverages
pharmaceutical
microbiology

suitability

Plesiomonas spp.
Aeromonas spp.
Neisseria spp.
Pseudomonas spp.
bacteria

Related Categories

General description

Oxidase Reagent, according to Gordon-McLeod, is a commonly used laboratory reagent in microbiology to detect bacteria that produce cytochrome c oxidase. The reagent is designed to identify this enzyme′s presence, which plays a critical role in the electron transport chain and indicates respiratory metabolism in bacteria. The Gordon-McLeod reagent contains N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride, as the active component in the reagent. When an organism produces cytochrome c oxidase, the reagent undergoes a color change reaction, turning from colorless to blue or purple. This color change is a positive result, indicating the presence of cytochrome c oxidase activity in the tested organism.

Application

Oxidase Reagent, according to Gordon-McLeod, is a commonly used laboratory technique in industrial microbiology to differentiate between bacterial strains based on their ability to produce cytochrome c oxidase. The Gaby-Hadley A reagent provides a simple and efficient way to identify oxidase-positive bacteria, such as Pseudomonas, Vibrio, and some Neisseria species, while producing negative results for organisms that lack cytochrome c oxidase activity. in the food, beverage, and pharmaceutical industries.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P342 + P311

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP0670
BCCM3467
BCCM5735
BCCK1992
BCCJ4482

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Articles

Media for Staphylococcus Aureus Detection

Chromogenic media enable the selective detection of S. aureus, which produce bluish-green colonies that are clearly differentiated from other species.

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.

Differentiation of Escherichia coli from coliforms

There are many other methods of detection to indicate the presence of E. coli. Review common tests and biochemical reactions for this contaminant.

Pseudomonas Media and Tests

An article concerning the detection, identification, differentiation, and cultivation of Pseudomonas species.

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Protocols

Enterobacteriaceae in Food

General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.

Listeria: Surviving Bacteria

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

食品中的肠杆菌

根据EN-ISO 8523:1991和EN-ISO 4832:1991分别对食品中肠杆菌进行检测和计数的一般指南。

李斯特菌：不可不防

近年来，李斯特菌病被定为重大的公共健康问题。这种病的主要感染对象是老年人、孕妇、新生儿和免疫功能低下者。

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