volume
10 μL
material
blunt tip stainless steel needle (point style 3)
size
32 ga (blunt tip)
feature
blunt tip
packaging
pkg of 1 ea
manufacturer/tradename
Hamilton 80351
needle L
7.5 cm
compatibility
for use with Carlo Erba Injectors
General description
On-Column injection is generally performed to avoid vaporization of samples. This method involves injecting the cold sample directly on the column. Using retention gaps allows autosamplers to use on-column sample injection method. In this system, a 32-gauge needle is inserted in a cool narrow bore injection valve and then into capillary column.
The fixed needle design of this Hamilton syringe minimizes dead volume. Stainless steel needle, 12.5 cm (for Agilent/HP injectors) or 7.5 cm (for Carlo Erba injectors) × 0.24 mm O.D. (32 ga.), 90° tip. Use with 0.32 mm I.D. or larger columns.
Application
It may be used in a study done to understand the variations of capillary on-column fixed needle injection system in gas chromatography.
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Hubschmann, Hans-Joachim
Handbook of GC-MS: Fundamentals and Applications, 27-Apr, 124-124 (2015)
On-column injector for capillary gas chromatography.
Peters, T. L., T. J. Nestrick, and L. L. Lamparski.
Analytical Chemistry, 54.11, 1893-1895 (1982)
Katharina Timper et al.
Cell reports, 25(2), 383-397 (2018-10-12)
Mitochondrial oxidative phosphorylation (OXPHOS) and substrate utilization critically regulate the function of hypothalamic proopiomelanocortin (POMC)-expressing neurons. Here, we demonstrate that inactivation of apoptosis-inducing factor (AIF) in POMC neurons mildly impairs mitochondrial respiration and decreases firing of POMC neurons in lean
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