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Merck
CN

222550

Nile Blue chloride

Dye content 85 %

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About This Item

Empirical Formula (Hill Notation):
C20H20ClN3O
CAS Number:
Molecular Weight:
353.85
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
Colour Index Number:
51180
MDL number:
Beilstein/REAXYS Number:
3923952
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form

powder

Quality Level

composition

Dye content, 85%

solubility

water: 1 mg/mL, opaque, blue to very deep blue

λmax

638 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34

InChI

1S/C20H19N3O.ClH/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;/h5-12,21H,3-4H2,1-2H3;1H

InChI key

SHXOKQKTZJXHHR-UHFFFAOYSA-N

Application

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.

Biochem/physiol Actions

Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0). Nile blue chloride has been used to detect DNA and lipids. In addition, it also helps to identify bacterial growth. Nile blue chloride also facilitates diagnosis of disease linked with amyloid accumulation.


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Effect of ?-casein on surface activity of Quillaja bark saponin at fluid/fluid interfaces
Wojciechowski K
Food Hydrocolloids, 34, 208-216 (2014)
R. W. Sabnis
Handbook of Acid-Base Indicators (2007)
M Oshiki et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 64(3), 747-753 (2011-11-22)
The present study was conducted (1) to develop a rapid quantification method of polyhydroxyalkanoates (PHA) concentration in activated sludge by Nile blue A staining and fluorescence measurement and (2) to perform on-line monitoring of PHA concentrations in activated sludge. Activated



Global Trade Item Number

SKUGTIN
222550-25G04061832338972
222550-5G04061838778079