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About This Item
Empirical Formula (Hill Notation):
C20H20ClN3O
CAS Number:
Molecular Weight:
353.85
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
Colour Index Number:
51180
MDL number:
Beilstein/REAXYS Number:
3923952
InChI
1S/C20H19N3O.ClH/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;/h5-12,21H,3-4H2,1-2H3;1H
SMILES string
[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34
InChI key
SHXOKQKTZJXHHR-UHFFFAOYSA-N
form
powder
composition
Dye content, 85%
solubility
water: 1 mg/mL, opaque, blue to very deep blue
λmax
638 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
Related Categories
Application
Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.
Biochem/physiol Actions
Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0). Nile blue chloride has been used to detect DNA and lipids. In addition, it also helps to identify bacterial growth. Nile blue chloride also facilitates diagnosis of disease linked with amyloid accumulation.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Food Hydrocolloids, 34, 208-216 (2014)
R. W. Sabnis
Handbook of Acid-Base Indicators (2007)
Julie Dutet et al.
Pharmaceutical research, 29(12), 3464-3474 (2012-08-08)
To estimate the in vitro ungual penetration depth of sodium fluorescein and nile blue chloride by laser scanning confocal microscopy. The depth, uniformity and pathways of penetration of both markers into human nail during passive and iontophoretic experiments were investigated.
Cai Wang et al.
Journal of economic entomology, 105(5), 1758-1765 (2012-11-20)
The consumption and food transfer efficiency of two commercially used termite bait materials, southern yellow pine wood and cardboard, and one potential bait material, maize (Zea mays L.) cob, were evaluated for use against the Formosan subterranean termite, Coptotermes formosanus
Zhiping Cui et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 83(1), 1-7 (2011-09-29)
A dual-wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was developed to detect chondroitin sulfate (CS) with nile blue sulfate (NBS). At pH 3.0-4.0 Britton-Robinson (BR) buffer medium, CS interacted with NBS to form an ion-association complex. As a result, the
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