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Merck
CN

222550

Nile Blue chloride

Dye content 85 %

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About This Item

Empirical Formula (Hill Notation):
C20H20ClN3O
CAS Number:
2381-85-3
Molecular Weight:
353.85
NACRES:
NA.47
PubChem Substance ID:
24853342
UNSPSC Code:
12171500
Colour Index Number:
51180
MDL number:
MFCD00011936
Beilstein/REAXYS Number:
3923952

Key Documents

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Product Name

Nile Blue chloride, Dye content 85 %

InChI

1S/C20H19N3O.ClH/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;/h5-12,21H,3-4H2,1-2H3;1H

SMILES string

[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34

InChI key

SHXOKQKTZJXHHR-UHFFFAOYSA-N

form

powder

composition

Dye content, 85%

solubility

water: 1 mg/mL, opaque, blue to very deep blue

λmax

638 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.

Biochem/physiol Actions

Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0). Nile blue chloride has been used to detect DNA and lipids. In addition, it also helps to identify bacterial growth. Nile blue chloride also facilitates diagnosis of disease linked with amyloid accumulation.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBQ1689
SHBN8805
SHBM3768
14527HA
03126CB

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