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Merck
CN

22310

Cereus Selective Agar

suitable for microbiology, NutriSelect® Basic

Synonym(s):

Bacillus Cereus Agar, Bacillus Cereus Selective Agar, Bacillus cereus Egg yolk Polymyxin Agar Base, Cereus Selective Agar Base acc. to Mossel, MYP Agar, Mannitol-Egg-yolk-polymyxine-Agar, PEMBA, PREP agar, Polymixin Pyruvate Egg yolk Mannitol Bromothymol blue Agar

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171606

Key Documents

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final pH

7.1±0.2 (25 °C)

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12 g/L , D(-)-mannitol, 10 g/L , meat extract, 1 g/L , meat peptone, 10 g/L , phenol red, 0.025 g/L , sodium chloride, 10 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

application(s)

clinical testing
environmental
food and beverages, microbiology

suitability

selective and differential for Bacillus cereus, selective and differential for Bacillus spp.

Quality Level

100

Related Categories

Application

For enumeration, identification and isolation of Bacillus cereus in food.

Preparation Note

Dissolve 43 g in 900 ml water. Sterilize by autoclaving at 121°C for 15 minutes and let cool to 45-50°C. Add 100 ml egg-yolk suspension (Cat. No. 17148) and 10 mg polymyxin B (Cat. No. 81334) or 2 vial/l Polymyxin B Selective Supplement (Cat. No. P9602) to the autoclaved solution.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM5129
BCCL5189
BCCK1052
BCCH1706
BCCF0207

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D A Mossel et al.
Applied microbiology, 15(3), 650-653 (1967-05-01)
For the enumeration of vegetative cells and spores of Bacillus cereus in foods, a mannitol-egg yolk-phenol red-agar has been developed which exploits the failure of B. cereus to dissimilate mannitol, and the ability of most strains to produce phospholipase C.

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