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Merck
CN

25328

4-Chloro-1-naphthol

≥99.0%

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About This Item

Linear Formula:
ClC10H6OH
CAS Number:
604-44-4
Molecular Weight:
178.61
UNSPSC Code:
12171500
NACRES:
MA.02
PubChem Substance ID:
329752790
EC Number:
210-068-5
Beilstein/REAXYS Number:
509750
MDL number:
MFCD00003974

Key Documents

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description

for analytical purposes

Quality Level

200

assay

≥99.0% (HPLC), ≥99.0%

mp

118-120 °C, 118-121 °C (lit.)

solubility

methanol: 50 mg/mL, clear, colorless to very faintly yellow

fluorescence

λex 310 nm; λem 375 nm in ethanol

SMILES string

Oc1ccc(Cl)c2ccccc12

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H

InChI key

LVSPDZAGCBEQAV-UHFFFAOYSA-N

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General description

4-Chloro-1-naphthol is primarily used for the detection of proteins, it gives a characteristic purple precipitate and the reactions are easily controllable. It can be used as a substitute for benzidine compounds, which are considered to be carcinogenic.

Application

4-chloro-1-naphthol along with N,N′-dimethyl-p-phenylendiamine may be used in an oxidative coupling reaction for visualization of antigen-antibody complexes on the nitrocellulose membrane. 4-Chloro-1-naphthol may also be used in scanning electrochemical microscopy (SECM), for imaging of DNA hybridization on microscopic polypyrrole patterns. It was used in immunoblotting analysis during purification of native specific proteins of Taenia crassiceps cysticerci antigens obtained by immunoaffinity chromatography.
electrophoresis test: suitable for peroxidase detection on blotting membranes

Other Notes

Peroxidase stain. In the detection of specific antigens after isoelectric focusing and immunoblotting into nitrocellulose membranes; In a dot-immunobinding assay for the detection and quantitation of human IgE; A rapid multicolor Western blot

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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N Lee et al.
Journal of immunological methods, 106(1), 27-30 (1988-01-21)
A multicolor Western blotting technique was developed, by which different kinds or different subtypes of interferon were identified with different colors on a single Western blot. This was achieved by sequentially applying different sets of probing antibodies, enzyme-conjugated developing antibodies
Endocrine Methods.
Meurant G.
Science, 214-215 (1996)
C S Hong et al.
Journal of immunological methods, 95(2), 195-202 (1986-12-24)
This paper describes a dot immunobinding assay for determining total human IgE with a tandem of monoclonal anti-IgE antibodies. Minute quantities of monoclonal anti-IgE antibodies were adsorbed on nitrocellulose discs. IgE bound to this solid phase monoclonal anti-IgE antibody was
L. Miribel et al.
Protides Biol. Fluid Proc. Colloq., 34, 753-753 (1986)
Noeli Maria Espíndola et al.
Journal of clinical microbiology, 43(7), 3178-3184 (2005-07-08)
Monoclonal antibodies (MAb) against Taenia crassiceps and Taenia solium cysticerci were produced and showed cross-reactivity with a 14-kDa protein from T. solium and with 18- and 14-kDa proteins from T. crassiceps. These MAbs and antibodies from cerebrospinal fluid (CSF) as
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