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Merck
CN

25668

Chloroform – isoamyl alcohol mixture

BioUltra, Molecular Biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Synonym(s):

Chloroform:Isoamyl Alcohol Solvent Mix

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About This Item

NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12352401
MDL number:
Assay:
≥99.5% (chloroform + isoamyl alcohol, GC)
Grade:
Molecular Biology
Technique(s):
GC/GC: suitable
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grade

Molecular Biology

Quality Level

product line

BioUltra

assay

≥99.5% (chloroform + isoamyl alcohol, GC)

form

liquid

technique(s)

GC/GC: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% free alkali (as NH3)

cation traces

Al: ≤0.5 mg/kg, Ba: ≤0.1 mg/kg, Bi: ≤0.1 mg/kg, Ca: ≤0.5 mg/kg, Cd: ≤0.05 mg/kg, Co: ≤0.02 mg/kg, Cr: ≤0.02 mg/kg, Cu: ≤0.02 mg/kg, Fe: ≤0.1 mg/kg, K: ≤0.5 mg/kg, Li: ≤0.1 mg/kg, Mg: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Mo: ≤0.1 mg/kg, Na: ≤0.5 mg/kg, Ni: ≤0.02 mg/kg, Pb: ≤0.1 mg/kg, Sr: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.1, λ: 280 nm Amax: 0.07

SMILES string

ClC(Cl)Cl.CC(C)CCO

InChI

1S/C5H12O.CHCl3/c1-5(2)3-4-6;2-1(3)4/h5-6H,3-4H2,1-2H3;1H

InChI key

BKHZIBWEHPHYAI-UHFFFAOYSA-N

Application

Chloroform – isoamyl alcohol mixture is a deproteinizing reagent used in conjunction with phenol for improving the efficiency of RNA and DNA (nucleic acid) extractions.

Other Notes

mixture of chloroform and isoamyl alcohol in the ratio of 49:1 (v/v)


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pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

易制毒化学品(2类)
危险化学品

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Purification of RNA by SDS solubilization and phenol extraction.
Rio DC, Ares M Jr, Hannon GJ, Nilsen TW.
Cold Spring Harbor Protocols, 2010, 5439-5439 (2010)
D.M. Wallace
Methods in Enzymology, 152, 35-35 (1987)
Bacterial RNA isolation.
Ares M.
Cold Spring Harbor Protocols, 1012, 1024-1027 (2012)



Global Trade Item Number

SKUGTIN
25668-100ML04061826058220
25668-500ML04061826058237
25668-15ML-KC04061838102881