29216
Thio-OMe tricesium salt
Synonym(s):
2-Amino-5-[5-(4-methoxyphenyl)-2-thienyl]phenol-N,N,O-triacetic acid tricesium salt
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About This Item
Empirical Formula (Hill Notation):
C23H18Cs3NO8S
Molecular Weight:
867.17
UNSPSC Code:
12352200
PubChem Substance ID:
MDL number:
SMILES string
[Cs+].[Cs+].[Cs+].COc1ccc(cc1)-c2ccc(s2)-c3ccc(N(CC([O-])=O)CC([O-])=O)c(OCC([O-])=O)c3
fluorescence
λex 337 nm; λem 418 nm in 0.1 M Tris pH 8.0, 10 mM Ca2+, λex 337 nm; λem 485 nm in 0.1 M Tris pH 8.0 (without Ca2+)
Features and Benefits
This is a new Calcium indicator with promising properties which provide an excellent addition to fluorescent Ca2+/Mg2+ indicators available already. It is a low-affinity Ca2+-indicators (Kd ca 126 μM). Such indicators are useful for determination of Ca2+ in intracellular compartments [or for monitoring steep increases in intracellular concentration of Ca2+ . The indicator offers the interesting property of being both excitation and emission rationable, thus ratiomeric measurements are possible both in excitations as emission mode.
Thio-OMe does also show strong blue shift of emission maximum (485nm to 418 nm) by binding to Ca2+.
Thio-OMe does also show strong blue shift of emission maximum (485nm to 418 nm) by binding to Ca2+.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
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