2B300
Sepharose™ 2B
60-200 μm bead diameter
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General description
Sepharose 2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
Application
Sepharose™ 2B has been used for the preparation of virosomes and platelets.
Legal Information
Sepharose is a trademark of Cytiva
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 2
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
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Regulation of the phosphoinositide cycle by Na+/H+ exchange and intracellular pH in human platelets.
G Luzzatto et al.
Biochimica et biophysica acta, 1084(1), 78-86 (1991-06-19)
We have found that thrombin-induced activation of protein kinase C (PKC) in platelets, measured by phosphorylation of the 47 kDa protein, is synergistically enhanced by the amiloride analogue ethylisopropylamiloride (EIA), a specific inhibitor of Na+/H+ exchange. This EIA effect was
Depletion of glycoprotein gp85 from virosomes made with Epstein-Barr virus proteins abolishes their ability to fuse with virus receptor-bearing cells.
Haddad RS and Hutt-Fletcher LM
Journal of Virology, 63, 4998-4998 (1989)
Balakrishnan Sivaraman et al.
Langmuir : the ACS journal of surfaces and colloids, 28(5), 2745-2752 (2011-12-24)
Recent studies have shown that platelets can adhere to adsorbed albumin (Alb) through a receptor-mediated mechanism, but only if the Alb undergoes more than a critical degree of adsorption-induced unfolding. The objectives of this research were to investigate whether Alb
M H Kroll et al.
Biochimica et biophysica acta, 970(1), 61-67 (1988-06-08)
Experiments were performed to elucidate the role of adenosine 3': 5'-cyclic monophosphate (cAMP) in the control of platelet protein kinase C (PKC) activation. Platelet aggregation and secretion in response to 4 beta-phorbol 12-myristate 13-acetate (PMA) or 1-oleoyl-2-acetylglycerol (OAG) were inhibited
Molecular Cloning and Physical Mapping of Potato Virus S Complementary DNA.
Monis, J., and de Zoeten, G.A.
Phytopathology, 80, 446-450 (1990)
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