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Merck
CN

30181

Fluorescein sodium salt

analytical standard

Synonym(s):

Acid Yellow 73, D&C;Yellow No. 8, NaFl, NaFluo, Sodium fluorescein, Uranine

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About This Item

Empirical Formula (Hill Notation):
C20H10Na2O5
CAS Number:
518-47-8
Molecular Weight:
376.27
EC Number:
208-253-0
UNSPSC Code:
85151701
NACRES:
NA.24
PubChem Substance ID:
329753341
MDL number:
MFCD00167039
Colour Index Number:
45350
Beilstein/REAXYS Number:
3833041

Key Documents

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Product Name

Fluorescein sodium salt, analytical standard

SMILES string

[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1

InChI key

RGPLGPBQJOQFJS-UHFFFAOYSA-L

InChI

1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2

grade

analytical standard

assay

≥97.5% (HPLC)

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

impurities

≤15% water

fluorescence

λex 460 nm; λem 515 nm(lit.)

format

neat

Quality Level

100

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Analysis Note

remarks on ICP: Na positive

Application

Fluorescein sodium salt is used as an analytical standard in determining the formation of hydroxy radicals, by the ZnO-based photocatalysts under UV-filtered simulated sunlight using spectrofluorometry. It is also used as a calibration standard for the label-free measurements of metabolic events within cells in the living animals using fluorescence lifetime imaging microscopy (FLIM).
It may also be used as an analytical reference standard for the quantification of the analyte in the following:
  • Soil samples using high-performance liquid chromatography (HPLC).
  • Coffee samples using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CZE).

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

General description

Fluorescein sodium salt (uranine) is a xanthene dye, which finds applications as a fluorescent tracer and shows the best of fluorescence in the pH range 6 to 11. Uranine is also employed as a coloring additive in drugs and cosmetics. It is known to exhibit optical chemical sensing and fluorescent properties in biological staining procedures.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

423.7 °F - Pensky-Martens closed cup

flash_point_c

217.6 °C - Pensky-Martens closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP1516
BCCK5605
BCCF5384
BCCC1797
BCBT6608

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Method for the analysis of phloxine B, uranine, and related xanthene dyes in soil using supercritical fluid extraction and high-performance liquid chromatography
Alcantara-Licudine PJ, et al.
Journal of Agricultural and Food Chemistry, 45(3), 766-773 (1997)
Phytochemical Characterization of Rhus coriaria L. Extracts by Headspace Solid-Phase Micro Extraction Gas Chromatography, Comprehensive Two-Dimensional Liquid Chromatography, and Antioxidant Activity Evaluation
Arena K, et al.
Molecules (Basel), 27(5), 1727-1727 (2022)
Jitendra Wankar et al.
International journal of pharmaceutics, 531(2), 568-576 (2017-05-27)
Ethionamide (ETH) is a second line antitubercular drug suffering from poor solubility in water and strong tendency to crystallize. These drawbacks were addressed by loading ETH in β-cyclodextrin (βCyD)-based carriers. The drug was incorporated in a molecular state avoiding crystallization
Analysis of phloxine B and uranine in coffee by high-performance liquid chromatography and capillary zone electrophoresis after solid phase extraction cleanup
Alcantara-Licudine PJ, et al.
Journal of Agricultural and Food Chemistry, 46(3), 1005-1011 (1998)
Highly reduced ecotoxicity of ZnO-based micro/nanostructures on aquatic biota: Influence of architecture, chemical composition, fixation, and photocatalytic efficiency
Serra A, et al.
Water Research, 169(9), 115210-115210 (2020)
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