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Merck
CN

30566

Trichloroacetic acid solution

suitable for DNA synthesis, 3 % (w/v) in methylene chloride

Synonym(s):

TCA

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About This Item

Linear Formula:
Cl3CCOOH
CAS Number:
76-03-9
Molecular Weight:
163.39
PubChem Substance ID:
24858446
UNSPSC Code:
12352200
Beilstein/REAXYS Number:
970119
MDL number:
MFCD00004177

Key Documents

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concentration

3 % (w/v) in methylene chloride

technique(s)

DNA synthesis: suitable

impurities

≤0.01% water

density

1.332 g/mL at 20 °C

SMILES string

OC(=O)C(Cl)(Cl)Cl

InChI

1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

YNJBWRMUSHSURL-UHFFFAOYSA-N

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General description

filtered through a 1 μm filter

Application

Reagent used in the phosphite triester method of oligonucleotide synthesis for the cleavage of the 5′-protect ing group. Reagent is suitable for use on ABI, Beckman, Expedite synthesizers.

Biochem/physiol Actions

Trichloroacetic acid solution is traditionally used to precipitate protein. It can be used to determine protein concentration by quantitative precipitation. Trichloroacetic acid can also be used as a decalcifier and fixative in microscopy.

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system, Respiratory system

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Regulatory Information

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