Skip to Content
Merck
CN

30911

Denaturation solution for the isolation of RNA

BioUltra, Molecular Biology

Synonym(s):

RNA isolation, denaturation

Sign In to View Organizational & Contract Pricing.

Select a Size


About This Item

UNSPSC Code:
12352202
NACRES:
NA.25
MDL number:
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

grade

Molecular Biology

product line

BioUltra

composition

guanidine thiocyanate, 4 M , Na-citrate, 25 mM , sarcosyl, 0.5%

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.433

pH

5.5-7.0

anion traces

chloride (Cl-): ≤500 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤2 mg/kg, As: ≤0.1 mg/kg, Ba: ≤2 mg/kg, Bi: ≤2 mg/kg, Ca: ≤10 mg/kg, Cd: ≤2 mg/kg, Co: ≤2 mg/kg, Cr: ≤2 mg/kg, Cu: ≤2 mg/kg, Fe: ≤2 mg/kg, K: ≤100 mg/kg, Li: ≤2 mg/kg, Mg: ≤2 mg/kg, Mn: ≤2 mg/kg, Mo: ≤2 mg/kg, Ni: ≤2 mg/kg, Pb: ≤2 mg/kg, Sr: ≤2 mg/kg, Zn: ≤2 mg/kg

λ

neat

UV absorption

λ: 280 nm Amax: 1

Quality Level

Application

Denaturation solution for the isolation of RNA is a guanidine thiocyanate, sarcosyl based protein denaturization solution useful for the purification of mRNA.

Other Notes

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

新产品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Y L Tsai et al.
Applied and environmental microbiology, 57(3), 765-768 (1991-03-01)
A protocol for direct extraction of mRNA from soil samples was developed. Soil samples (10 g) were washed twice with 120 mM phosphate buffer (pH 5.2). The lysis of cells, fixation of RNA, and hydrolysis of DNA were achieved by
P Chomczynski et al.
Analytical biochemistry, 162(1), 156-159 (1987-04-01)
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service