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Merck
CN

33731

Trichloroacetic acid

puriss. p.a., ACS reagent, ≥99.5%

Synonym(s):

TCA

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About This Item

Linear Formula:
Cl3CCOOH
CAS Number:
Molecular Weight:
163.39
EC Number:
200-927-2
UNSPSC Code:
12352100
PubChem Substance ID:
Beilstein/REAXYS Number:
970119
MDL number:
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vapor density

<1 (vs air)

vapor pressure

1 mmHg ( 51 °C)

grade

ACS reagent, puriss. p.a.

assay

≥99.5%

impurities

≤0.002% heavy metals (as Pb), ≤0.005% water insolubles

ign. residue

≤0.01% (as SO4)

refractive index

n20/D 1.62 (lit.)

bp

196 °C (lit.)

mp

54-58 °C (lit.)

density

1.62 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤10 mg/kg, nitrate (NO3-): ≤20 mg/kg, phosphate (PO43-): ≤5 mg/kg, sulfate (SO42-): ≤100 mg/kg

cation traces

Cu: ≤5 mg/kg, Fe: ≤10 mg/kg

suitability

complies for appearance of solution

SMILES string

OC(=O)C(Cl)(Cl)Cl

InChI

1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

YNJBWRMUSHSURL-UHFFFAOYSA-N

General description

Trichloroacetic acid (TCA) is a chlorinated acetic acid. It is one of the major metabolite of trichloroethylene (TCE).

Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.
Trichloroacetic acid may be used for the quantification of inorganic phosphorus in various biological samples (serum, urine and stool ash solutions). It may be used for the elimination of acid-soluble and lipid phosphorous during the quantitative estimation of deoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissues. 10% trichloroacetic acid may be employed for the preparation of radioactive proteins.


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pictograms

CorrosionEnvironment

signalword

Danger

hcodes

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

>235.4 °F - closed cup

flash_point_c

> 113 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Information

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A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues.
G SCHMIDT et al.
The Journal of biological chemistry, 161, 83-89 (1945-01-01)
Solubility of albumin in alcohol after precipitation by trichloroacetic acid: a simplified procedure for separation of albumin.
J R DEBRO et al.
Nature, 178(4541), 1067-1067 (1956-11-10)
A microcolorimetric method for the determination of inorganic phosphorus.
H H TAUSSKY et al.
The Journal of biological chemistry, 202(2), 675-685 (1953-06-01)