33871
2-Nitrobenzaldehyde semicarbazone
VETRANAL®, analytical standard
Synonym(s):
2-NP-SCA, 4-(2-Nitrobenzylidene)semicarbazide
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About This Item
Empirical Formula (Hill Notation):
C8H8N4O3
CAS Number:
Molecular Weight:
208.17
NACRES:
NA.24
PubChem Substance ID:
UNSPSC Code:
41116107
MDL number:
InChI
1S/C8H8N4O3/c9-8(13)11-10-5-6-3-1-2-4-7(6)12(14)15/h1-5H,(H3,9,11,13)/b10-5+
SMILES string
NC(=O)N\N=C\c1ccccc1[N+]([O-])=O
InChI key
OEOKLBSECDAYSM-BJMVGYQFSA-N
grade
analytical standard
product line
VETRANAL®
shelf life
limited shelf life, expiry date on the label
technique(s)
HPLC: suitable, gas chromatography (GC): suitable
application(s)
clinical testing
format
neat
Quality Level
General description
2-Nitrobenzaldehyde semicarbazone is a semicarbazone derivative of 2-Nitrobenzaldehyde.
Application
2-Nitrobenzaldehyde semicarbazone (2-NBA-SC) may be used as an analytical reference standard for the determination of 2-NBA-SC in:
- Animal muscle tissues by solid-phase extraction (SPE) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) equipped with electrospray ionization (ESI) source and multiple reaction monitoring (MRM) detection.
- Milk of dairy cows by LC-MS/MS with selected reaction monitoring (SRM) detection.
- Azodicarbonamide-treated flour samples by isotope dilution liquid chromatography coupled to ESI-LC-MS/MS.
- Shrimp dosed with nitrofurans by ESI-LC-MS/MS with SRM detection.
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Other Notes
Derivatized standard for the assay of nitrofurans in animal tissue by LC/MS
Legal Information
VETRANAL is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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Microwave-assisted Synthesis and Bioevaluation of Some Semicarbazones.
Jafri L, et al.
Chemical Biology & Drug Design, 79(6), 950-959 (2012)
Liquid chromatography- tandem mass spectrometry for the determination of protein-bound residues in shrimp dosed with nitrofurans.
Chu PS and Lopez MI
Journal of Agricultural and Food Chemistry, 53(23), 8934-8939 (2005)
Determination of the metabolites of nitrofuran antibiotics in animal tissue by high-performance liquid chromatography-tandem mass spectrometry.
Leitner A, et al.
Journal of Chromatography A, 939(1-2), 49-58 (2001)
Adam Becalski et al.
Journal of agricultural and food chemistry, 52(18), 5730-5734 (2004-09-18)
Semicarbazide was previously found in foods that were in contact with rubber gaskets foamed at high temperatures with a blowing agent azodicarbonamide. Because azodicarbonamide is an approved flour additive in certain countries, we set out to ascertain if semicarbazide is
Determination of nitrofuran residues in milk of dairy cows using liquid chromatography- tandem mass spectrometry.
Chu PS and Lopez MI
Journal of Agricultural and Food Chemistry, 55(6), 2129-2135 (2007)
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