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Merck
CN

39727

Perfringens Agar Base

suitable for microbiology, NutriSelect® Plus

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171609
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Product Name

Perfringens Agar Base, suitable for microbiology, NutriSelect® Plus

sterility

non-sterile

form

powder

composition

Agar, 15 g/L
Beef extract, 5 g/L
Ferric ammonium citrate, 1 g/L
Papaic digest of soybean meal, 5 g/L
Sodium metabisulphite, 1 g/L
Tryptose, 15 g/L
Yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

application(s)

bioburden testing
cleaning products
cosmetics
environmental
food and beverages
personal care

microbiology

suitability

selective and differential for Clostridium spp.

Quality Level

Application

Perfringens Agar Base has been used in the cell culture of Clostridium perfringens.

General description

Perfringens Agar Base with the addition of selective supplement and enrichment is used for the presumptive identification and enumeration of Clostridium perfringens from food.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 23.5 g of Perfringens Agar Base in 475 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C. Add 25 ml of Egg Yolk Emulsion (Cat. No. 17148) and additionally the rehydrated contents of 1 vial of Perfringens S.F.P. Supplement (Cat. No. 53436) or 1 vial of Perfringens T.S.C. Supplement (Cat. No. P9352). Mix well and pour into sterile petri plates.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Martina Hustá et al.
Anaerobe, 63, 102205-102205 (2020-05-01)
Isolation and fast detection of Clostridium perfringens is essential in veterinary medical diagnostics and veterinary research, as it allows to recommend suitable treatment options after antimicrobial resistance determination, and is essential to study pathogenesis. In this study four selective media

Articles

Clostridium perfringens can cause contamination in undercooked or improperly sterilized canned foods and water. Learn to detect, identify, and differentiate this pathogen.

产气荚膜梭菌会导致未煮熟或未正确消毒的罐头食品和水受到污染。了解如何检测、鉴定和区分这种病原体。

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