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Merck
CN

424455

Resorufin

Dye content 95 %

Synonym(s):

7-Hydroxy-3H-phenoxazin-3-one, NSC 12097

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About This Item

Empirical Formula (Hill Notation):
C12H7NO3
CAS Number:
635-78-9
Molecular Weight:
213.19
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24866568
EC Number:
211-241-8
Beilstein/REAXYS Number:
174850
MDL number:
MFCD00128991

Key Documents

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Product Name

Resorufin, Dye content 95 %

InChI key

HSSLDCABUXLXKM-UHFFFAOYSA-N

InChI

1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H

SMILES string

O=C(C=C1)C=C2C1=NC3=CC=C(O)C=C3O2

form

powder

composition

Dye content, 95%

mp

>300 °C (lit.)

λmax

573 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Resorufin has been used in the measurements of hydrogen peroxide generation.

General description

Resorufin, a redox probe can be used to visualize cell respiration directly. It is highly fluorescent and has an excitation maximum at 563nm and emission maximum at 587nm.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKBT9342V
MKBN9354V
MKBB5090
41997MJ
11128CE

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Hubert M Piwonski et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(22), E1437-E1443 (2012-05-09)
Enzymatic inhibition by product molecules is an important and widespread phenomenon. We describe an approach to study product inhibition at the single-molecule level. Individual HRP molecules are trapped within surface-tethered lipid vesicles, and their reaction with a fluorogenic substrate is
Yutaka Hitomi et al.
Analytical chemistry, 83(24), 9213-9216 (2011-11-18)
We developed a metal-based fluorescent probe for H(2)O(2) called MBFh1, which has an iron complex as a reaction site for H(2)O(2) and a 3,7-dihydroxyphenoxazine derivative as the fluorescent reporter unit. The iron complex reacts quickly with H(2)O(2) to form oxidants
Eduardo Vottero et al.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 16(6), 899-912 (2011-05-14)
CYP102A1, originating from Bacillus megaterium, is a highly active enzyme which has attracted much attention because of its potential applicability as a biocatalyst for oxidative reactions. Previously we developed drug-metabolizing mutant CYP102A1 M11 by a combination of site-directed and random
Malena B Rone et al.
Molecular endocrinology (Baltimore, Md.), 26(11), 1868-1882 (2012-09-14)
Steroid hormones are critical for organismal development and health. The rate-limiting step in steroidogenesis is the transport of cholesterol from the outer mitochondrial membrane (OMM) to the cytochrome P450 enzyme CYP11A1 in the inner mitochondrial membrane (IMM). Cholesterol transfer occurs
Julian G Jude et al.
Oncogene, 34(10), 1253-1262 (2014-04-01)
Given the importance of deregulated phosphoinositide (PI) signaling in leukemic hematopoiesis, genes coding for proteins that regulate PI metabolism may have significant and as yet unappreciated roles in leukemia. We performed a targeted knockdown (KD) screen of PI modulator genes
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