Müller-Kauffmann Tetrathionate Broth, Base (ISO)

Müller-Kauffmann Tetrathionate Broth, Base (ISO) is a selective enrichment of Salmonellae from various sources like milk and other food items.

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Suspend 107 g of powder in 1 litre of distilled water, mix well and bring to the boil and let it cool to 40-45°C. Immediately before use add 20 ml of iodine-iodide solution prepared by dissolving 5 g of potassium iodide in 10 ml of water, adding 6 g of iodine and then diluting to 20 ml with sterile water. Also add the contents of two vials of Brilliant Green Novobiocin Selective Supplement (Cat. No. 55181) reconstituted as directed. Mix well and aseptically dispense into sterile tubes. Do not heat after adding the iodine solution. Complete medium must be used immediately; the base, without iodine, may be stored in the refrigerator for some days. White precipitate is due to calcium carbonate and is must be considered as normal.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany