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Merck
CN

43623

β-Sitosterol

analytical standard

Synonym(s):

α-Dihydrofucosterol, 22,23-Dihydrostigmasterol, 24α-Ethylcholesterol, 5-Stigmasten-3β-ol

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About This Item

Empirical Formula (Hill Notation):
C29H50O
CAS Number:
83-46-5
Molecular Weight:
414.71
UNSPSC Code:
12352104
NACRES:
NA.24
PubChem Substance ID:
329756360
EC Number:
201-480-6
Beilstein/REAXYS Number:
1916165
MDL number:
MFCD00003631

Key Documents

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technique(s)

gas chromatography (GC): suitable, HPLC: suitable

SMILES string

O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CC[C@H](C(C)C)CC

InChI key

KZJWDPNRJALLNS-VJSFXXLFSA-N

InChI

1S/C29H50O/c1-7-21(19(2)3)9-8-20(4)25-12-13-26-24-11-10-22-18-23(30)14-16-28(22,5)27(24)15-17-29(25,26)6/h10,19-21,23-27,30H,7-9,11-18H2,1-6H3/t20-,21-,23+,24+,25-,26+,27+,28+,29-/m1/s1

grade

analytical standard

assay

≥90.0% (GC)

shelf life

limited shelf life, expiry date on the label

impurities

≤10.0% water

mp

136-140 °C (lit.)

application(s)

food and beverages

format

neat

storage temp.

2-8°C

Quality Level

100

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General description

β-Sitosterol is an abundantly found phytosterol in plants. It is known to show anti-inflammatory, immune-modulating, antipyretic, antineoplastic, and blood sugar-controlling properties. β-Sitosterol also acts as an estrogen and estrogen receptor (ERα and Erβ) agonist.

Application

This analytical standard can also be used as follows:
  • Identification and determination of β-sitosterol and stigmasterol in the hydro-alcohol extract, prepared from the leaves of B. bambo by reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to photo-diode array detector (PDA)
  • Development and validation of two high-performance thin layer chromatography (HPTLC) methods to quantify four biomarkers— β-sitosterol, β-amyrin, lupeol, and ursolic acid in the ethanol extract of G. senegalensis leaves
  • Secondary metabolite profiling of various plant parts collected from 82 plants belonging to 21 different cannabis strains using gas chromatography-mass spectrometry (GC-MS) for sterols and terpenoids (mono-, sesqui-, tri-), and high-performance liquid chromatography (HPLC) with UV and mass spectrometric (MS) detection for flavonoids
  • High-performance thin layer chromatographic (HPTLC) method-based quantification of β-sitosterol in a herbal formulation prepared from the roots of Gmelina arborea using silica gel precoated 60 F254 aluminum plates
  • Determination of free sterols or oxysterols in food matrices, using gas chromatography and ion trap mass spectrometry (GC-MS).

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCJ6836
BCCH6799
BCCH2886
BCCH0755
BCCG9032

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?-Sitosterol activates Fas signaling in human breast cancer cells
Awad.B.A, et al.
Phytomedicine, 14, 747-754 (2007)
New method to determine free sterols/oxysterols in food matrices using gas chromatography and ion trap mass spectrometry (GC?IT-MS)
Szterk A and Pakula L
Talanta, 152, 54-75 (2016)
Ashutosh Singh
Food chemistry, 137(1-4), 62-67 (2012-12-04)
The antioxidative effect of sitosterol at 1, 2 and 5% levels, in triolein, refined canola, high oleic sunflower and flaxseed oils, continuously heated for a period of up to 72 h at frying temperature of 180 °C, was studied. High
Günther Silbernagel et al.
Current opinion in lipidology, 24(1), 12-17 (2012-11-21)
Plant sterols as ingredients to functional foods are recommended for lowering LDL cholesterol. However, there is an ongoing discussion whether the use of plant sterols is safe. Genetic analyses showed that common variants of the ATP binding cassette transporter G8
M A Nurseitova et al.
Journal of dairy science, 102(9), 7723-7733 (2019-07-01)
Milk fat adulteration is a common issue in Central Asia. To assess the current situation in the commercial milk market, 17 milk samples were checked for fatty acid (FA) and sterol profiles to detect potential adulteration using multivariate analysis. Analysis
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