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Merck
CN

43623

β-Sitosterol

analytical standard

Synonym(s):

α-Dihydrofucosterol, 22,23-Dihydrostigmasterol, 24α-Ethylcholesterol, 5-Stigmasten-3β-ol

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About This Item

Empirical Formula (Hill Notation):
C29H50O
CAS Number:
Molecular Weight:
414.71
UNSPSC Code:
12352104
NACRES:
NA.24
PubChem Substance ID:
EC Number:
201-480-6
Beilstein/REAXYS Number:
1916165
MDL number:
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grade

analytical standard

Quality Segment

assay

≥90.0% (GC)

shelf life

limited shelf life, expiry date on the label

technique(s)

gas chromatography (GC): suitable, HPLC: suitable

impurities

≤10.0% water

mp

136-140 °C (lit.)

application(s)

food and beverages

format

neat

storage temp.

2-8°C

SMILES string

O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CC[C@H](C(C)C)CC

InChI

1S/C29H50O/c1-7-21(19(2)3)9-8-20(4)25-12-13-26-24-11-10-22-18-23(30)14-16-28(22,5)27(24)15-17-29(25,26)6/h10,19-21,23-27,30H,7-9,11-18H2,1-6H3/t20-,21-,23+,24+,25-,26+,27+,28+,29-/m1/s1

InChI key

KZJWDPNRJALLNS-VJSFXXLFSA-N

General description

β-Sitosterol is an abundantly found phytosterol in plants. It is known to show anti-inflammatory, immune-modulating, antipyretic, antineoplastic, and blood sugar-controlling properties. β-Sitosterol also acts as an estrogen and estrogen receptor (ERα and Erβ) agonist.

Application

This analytical standard can also be used as follows:
  • Identification and determination of β-sitosterol and stigmasterol in the hydro-alcohol extract, prepared from the leaves of B. bambo by reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to photo-diode array detector (PDA)
  • Development and validation of two high-performance thin layer chromatography (HPTLC) methods to quantify four biomarkers— β-sitosterol, β-amyrin, lupeol, and ursolic acid in the ethanol extract of G. senegalensis leaves
  • Secondary metabolite profiling of various plant parts collected from 82 plants belonging to 21 different cannabis strains using gas chromatography-mass spectrometry (GC-MS) for sterols and terpenoids (mono-, sesqui-, tri-), and high-performance liquid chromatography (HPLC) with UV and mass spectrometric (MS) detection for flavonoids
  • High-performance thin layer chromatographic (HPTLC) method-based quantification of β-sitosterol in a herbal formulation prepared from the roots of Gmelina arborea using silica gel precoated 60 F254 aluminum plates
  • Determination of free sterols or oxysterols in food matrices, using gas chromatography and ion trap mass spectrometry (GC-MS).

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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