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Merck
CN

43665

Direct Red 80

Synonym(s):

Sirius Red

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About This Item

Empirical Formula (Hill Notation):
C45H26N10Na6O21S6
CAS Number:
2610-10-8
Molecular Weight:
1373.07
EC Number:
220-027-3
PubChem Substance ID:
24866989
UNSPSC Code:
12171500
Beilstein/REAXYS Number:
8185575
Colour Index Number:
35780
MDL number:
MFCD00054389

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InChI

1S/C45H32N10O21S6.6Na/c56-43-33-13-5-27(17-23(33)19-39(81(71,72)73)41(43)54-52-35-15-7-29(21-37(35)79(65,66)67)50-48-25-1-9-31(10-2-25)77(59,60)61)46-45(58)47-28-6-14-34-24(18-28)20-40(82(74,75)76)42(44(34)57)55-53-36-16-8-30(22-38(36)80(68,69)70)51-49-26-3-11-32(12-4-26)78(62,63)64;;;;;;/h1-22,56-57H,(H2,46,47,58)(H,59,60,61)(H,62,63,64)(H,65,66,67)(H,68,69,70)(H,71,72,73)(H,74,75,76);;;;;;/q;6*+1/p-6/b50-48+,51-49+,54-52+,55-53+;;;;;;

InChI key

YIQKLZYTHXTDDT-IJPLDVMMSA-H

SMILES string

[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c(cc4cc(NC(=O)Nc5ccc6c(O)c(\N=N\c7ccc(cc7S([O-])(=O)=O)\N=N\c8ccc(cc8)S([O-])(=O)=O)c(cc6c5)S([O-])(=O)=O)ccc14)S([O-])(=O)=O

suitability

suitable for microscopy

Other Notes

Stain used to demonstrate collagenous septa (picrosirius red technique)
This product has been replaced by 365548-Sigma-Aldrich | Direct Red 80 Dye content 25 %

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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G E Piérard
Matrix (Stuttgart, Germany), 9(1), 68-71 (1989-01-01)
There are several reports in the literature where the sirius red polarization method is used to identify the molecular type of collagen in tissue sections. We present evidence that such way of interpretation is erroneous. The sirius red polarization method
SIRIUS RED F3BA AS A STAIN FOR CONNECTIVE TISSUE.
F SWEAT et al.
Archives of pathology, 78, 69-72 (1964-07-01)
P C Dolber et al.
Stain technology, 62(1), 23-26 (1987-01-01)
When the picrosirius red technique was applied to cardiac muscle sections, intense yellow myocyte staining sometimes obscured thin collagenous septa. The picrosirius red technique was modified to include treatment of the sections in 0.2% (w/v) aqueous phosphomolybdic acid prior to
Christopher A Miller et al.
Circulation. Cardiovascular imaging, 6(3), 373-383 (2013-04-05)
Extracellular matrix expansion is a key element of ventricular remodeling and a potential therapeutic target. Cardiovascular magnetic resonance (CMR) T1-mapping techniques are increasingly used to evaluate myocardial extracellular volume (ECV); however, the most widely applied methods are without histological validation.
Corrine R Kliment et al.
International journal of clinical and experimental pathology, 4(4), 349-355 (2011-05-18)
The purpose of this study was to develop an improved method for collagen and protein assessment of fibrotic lungs while decreasing animal use. 8-10 week old, male C57BL/6 mice were given a single intratracheal instillation of crocidolite asbestos or control
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