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459836

Sigma-Aldrich

Ethyl alcohol, Pure

greener alternative

200 proof, anhydrous, ≥99.5%

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Synonym(s):
Ethanol, absolute alcohol, non-denatured ethanol
Linear Formula:
CH3CH2OH
CAS Number:
Molecular Weight:
46.07
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

grade

anhydrous

Quality Level

vapor density

1.59 (vs air)

Assay

≥99.5%

form

liquid

expl. lim.

3.1-27.7 %

availability

not available in (Sales restictions may apply)

greener alternative product characteristics

Safer Solvents and Auxiliaries
Use of Renewable Feedstocks
Learn more about the Principles of Green Chemistry.

concentration

200 proof

impurities

<0.005% water

evapn. residue

<0.0005%

color

colorless

pH

7 (20 °C, 10 g/L)

bp

78.3 °C

mp

-114 °C

density

0.789 g/mL at 20 °C

greener alternative category

InChI

1S/C2H6O/c1-2-3/h3H,2H2,1H3

InChI key

LFQSCWFLJHTTHZ-UHFFFAOYSA-N

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General description

Pure ethyl alcohol is a non-denatured 200proof ethanol. Ethanol, a primary alcohol, is an important raw material for the synthesis of various compounds. It can be synthesized either from molasses by fermentation or by hydration of ethylene. It acts as a hydrogen source and reaction medium during the transfer hydrogenation of carbonyl compounds in the presence of sodium hydroxide. The impact of ethanol/water fumigation on the performance of diesel engines has been studied. Anhydrous grade solvents show extremely low percentage water and low residue levels.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. Ethanol is environmentally preferable green solvent and thus it aligns with "Safer Solvents and Auxiliaries" and "Use of Renewable Feedstocks". 

Application

Ethanol has been used:
  • as a solvent for lovastatin.
  • as a solvent during the synthesis of (S)-4-hydroxy-2-keto-4-(2′-pyridyl)butyrate.
  • to fix tissue sections on a slide during laser capture microdissection (LCM).

Other Notes

Pure-Pac® II containers require the Micromatic MacroValve coupler for dispensing solvents, Z560723.
Sales restrictions may apply

Legal Information

Pure-Pac is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

55.4 °F

Flash Point(C)

13 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品

Certificates of Analysis (COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Who do I contact about larger solvent volume needs?

    Email us at labessentials@sial.com or visit Sigma-Aldrich Fine Chemicals for development and manufacturing-scale inquiries: safcglobal@sial.com

  4. How do I know what packaging options are available for a solvent?

    The Solvent Center gives you the ability to review all the specifications associated with our solvent packaging. This includes physical dimensions, closure types, dispensing methods, UN/DOT Rating as well as solvent compatibility. Visit the Solvent Center.

  5. Who do I contact if I need a solvent blend, which you do not carry?

    Email us at labessentials@sial.com. For a complete listing of all our Solvent blends, Visit the Solvent Center

  6. The dispensing of solvents provides some challenges due to the number of container options available. What dispensing options are available for each container?

    The Sigma-Aldrich Solvent Center provides numerous dispensing options for each container type. For a complete listing of all our dispensing options, Visit the Solvent Center.

  7. Which solvent grade should I be using for my application?

    Sigma-Aldrich Solvent Applications Table gives a guideline to common applications and the appropriate solvent grades.  Visit: Solvents

  8. What are the options for drying solvents? 

    Sigma-Aldrich offers a full range of high-purity solvents with extremely low water levels specifically manufactured for moisture sensitive Organic and Biotech applications.  Sigma-Aldrich also carries various drying agents such as molecular sieves, which are typically compatible with organic solvents.

  9. What apparatus do you recommend for HPLC solvent filtration?

    The Sigma-Aldrich Vacuum Filtration Assembly gives you all the components you need including: funnel top, fritted glass funnel support, filtration flask, aluminum clamp, and silicone stopper.  The Acrodisc Syringe Filters offer high quality filtration for analytical samples, certified for HPLC to ensure low extractable and available in a broad range of membranes to meet sample compatibility requirements.

  10. Can you recommend a Solvent Extractor for organics from aqueous solution?

    Solvent extractors are often used for the quantitative extraction of organics from aqueous solutions and separations in immunoassays. The MIXXOR system has been applied successfully in many laboratory solvent extraction operations and is ideal for the rapid screening of alternative solvents for specific extraction problems.

  11. What is 190 proof and 200 proof ethanol?

    All non-denatured ethanol products have a proof associated with the product description. The proof is a measure of the water content of the ethanol portion of the product. The proof is calculated as two times (2 x) the actual ethanol concentration by volume.

  12. How can I get help with the ATF paperwork for 190 and 200 proof ethanol?

    Because these non-denatured ethanol products are federally regulated, an ATF permit is required to purchase 190 and 200 proof ethanol. Sigma-Aldrich takes the hassle out of this process for you! There is no ATF paperwork needed or records to be kept on your part. Sigma-Aldrich holds the permit, and immediately pays the federal excise tax. For more regulatory information regarding the tax on ethanol, visit the U.S. Department of Justice, Bureau of Alcohol, Tobacco, Firearms and Explosives.

  13. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  14. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  15. Does Product 459836, Ethanol, contain any denaturants?

    This product does not contain any denaturants. A denaturant is an impurity (e.g. isopropyl alcohol, methyl alcohol, etc.) that is added to ethanol to make it unsuitable for consumption. Even though this ethanol is not denatured, it is still only suitable for research and development use and is not appropriate for human use products.

  16. Is Product 459836, Ethanol, DNase, RNase, and Protease free?

    This product is not tested for the presence of DNases, RNases, and proteases. However, Sigma-Aldrich does offer ethanol products that are tested for these impurities. Ethanol products that are part of the BioUltra product line, and for applications in molecular biology, are required to be DNase, RNase, protease, and phosphatase free.

  17. What are the options for drying solvents?

    Sigma-Aldrich offers a full range of high-purity solvents with extremely low water levels specifically manufactured for moisture sensitive organic and biotech applications.  Sigma-Aldrich also carries various drying agents, which are compatible with organic solvents. For drying ethanol, 3Å molecular sieves are a useful medium for removing any remaining water. For example, we offer these drying agents under such product numbers as 208574 and 208582.

  18. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

J Harry Sweigard et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 28(7), 3171-3182 (2014-03-29)
A defining feature in proliferative retinopathies is the formation of pathological neovessels. In these diseases, the balance between neovessel formation and regression determines blindness, making the modulation of neovessel growth highly desirable. The role of the immune system in these
Sodium hydroxide-catalyzed transfer hydrogenation of carbonyl compounds and nitroarenes using ethanol or isopropanol as both solvent and hydrogen donor.
Wang D, et al.
J. Mol. Catal. A: Chem., 400, 14-21 (2015)
Sukant Khurana et al.
Chemical senses, 38(4), 315-323 (2013-02-01)
We studied complete dose-response curves for 53 odorants in the third instar larvae of Drosophila melanogaster. All odorants, except one, elicited an attraction response. Some odorants also elicited a decrease from their peak response at higher concentrations. This concentration-dependent decrease
Matthew J Walters et al.
Nature protocols, 2(7), 1825-1830 (2007-07-21)
A procedure for the preparation of optically pure alpha-keto-gamma-hydroxy carboxylic acids through stereospecific aldol addition catalyzed by pyruvate aldolases from the Entner-Doudoroff and the DeLey-Doudoroff glycolytic pathways is described. This highly versatile fragment serves as a precursor for a variety
Assessment of a direct injection diesel engine fumigated with ethanol/water mixtures.
Morsy MH.
Energy Conversion and Management , 94, 406-414 (2015)

Articles

Development and validation of a headspace SPME and GC-MS method to determine alcohol content in twenty different Kombucha tea samples.

Protocols

This protocol is designed to allow for high throughput purification of pure genomic DNA from a variety of cultured cells and tissues using the GenElute 96 Well Tissue Genomic DNA Purification Kit.

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

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