48281
β-1,4-Galactosyl Transferase from bovine milk
lyophilized, powder, ~8 U/g
Synonym(s):
Lactose Synthase
form
powder
quality
lyophilized
specific activity
~8 U/g
storage temp.
−20°C
Other Notes
1 U correspond to the amount of enzyme which transfers 1 μmol galactose from UDP-galactose to D-glucose per minute at pH 8.4 and 30°C in the presence of α-lactalbumin
Catalyst for the chemoenzymatic oligosaccharide synthesis, e.g. transfer of galactose
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S.I. Nishimura et al.
Tetrahedron Letters, 35, 5657-5657 (1994)
Y. Nishida et al.
Tetrahedron Letters, 33, 8043-8043 (1992)
O. Seitz et al.
Journal of the American Chemical Society, 119, 8766-8766 (1997)
Y Kajihara et al.
Carbohydrate research, 269(2), 273-294 (1995-04-19)
UDP-6-Deoxy-D-galactose and UDP-6-deoxy-6-fluoro-D-galactose were synthesized and their transfer to 2-acetamido-2-deoxy-D-glucose (N-acetyl-D-glucosamine) by beta-(1-->4)-galactosyltransferase was examined. The transfer rates of 6-deoxy-D-galactose and 6-deoxy-6-fluoro-D-galactose were 1.3 and 0.2% of that of D-galactosyl transfer, respectively. The 2-acetamido-4-O-(6-deoxy-beta-D-galactopyranosyl)-2-deoxy-D-glucopyranose (6'-deoxy-N-acetyllactosamine) and methyl 2-acetamido-4-O-(6-deoxy-6-fluoro-beta-D-galactopyranosyl)-2-deoxy-D- glucopyranoside (6'-deoxy-6'-fluoro-N-acetyllactosamine)
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